Banks W A, Kastin A J
Department of Veterans Affairs, New Orleans, LA.
Life Sci. 1991;48(25):PL117-21. doi: 10.1016/0024-3205(91)90385-o.
Interleukins (IL) are naturally occurring proteins that regulate, and thus link, both the immune system and the central nervous system (CNS). Since proteins are assumed not to be able to cross the blood-brain barrier (BBB), it is controversial how this linkage could occur. We show here that after iv injection of 125I-hIL-1 alpha, radioactivity in the brain eluted on HPLC in the position of the labeled cytokine. In addition, entry was inhibited by unlabeled hIL-1 alpha. Our demonstration of a saturable, carrier-mediated system that transports recombinant human IL-1 alpha in intact form from the blood into the CNS indicates a direct immune-CNS connection.
白细胞介素（IL）是天然存在的蛋白质，可调节免疫系统和中枢神经系统（CNS），从而建立两者之间的联系。由于蛋白质被认为无法穿过血脑屏障（BBB），这种联系如何发生存在争议。我们在此表明，静脉注射125I-hIL-1α后，脑中的放射性在高效液相色谱（HPLC）上于标记细胞因子的位置洗脱。此外，未标记的hIL-1α可抑制其进入。我们证明了一种可饱和的、载体介导的系统，该系统可将完整形式的重组人IL-1α从血液转运至中枢神经系统，这表明免疫系统与中枢神经系统之间存在直接联系。
