Department of Dermatology, Division of Brain Korea 21 Project for Biomedical Science, Korea University College of Medicine, Seoul, Korea.
Int J Dermatol. 2010 Feb;49(2):158-61. doi: 10.1111/j.1365-4632.2009.04210.x.
Egr-1 is known to play an important role in cell growth, differentiation, and cell survival. Human skin dermal fibroblasts (HSDF) may be an important target for adverse effects of tobacco components. However, nicotinic effect for early growth response-1 (Egr-1) expression is unknown in HSDF.
Cytotoxicity of nicotine was assessed by cell viability test in HSDF. The expression of Egr-1 protein and mRNA after nicotine treatment was evaluated by Western blotting and real-time reverse transcription-polymerase chain reaction. We also measured the promoter activity of Egr-1 in HSDF after nicotine exposure.
Early growth response-1 protein and mRNA levels were increased in dermal fibroblasts exposed to nicotine. Nicotine treatment stimulated the promoter activity of Egr-1 in cultured human fibroblasts.
In this study, we demonstrate that Egr-1 expression is markedly induced in HSDF after exposure to nicotine.
Egr-1 已知在细胞生长、分化和细胞存活中发挥重要作用。人皮肤真皮成纤维细胞（HSDF）可能是烟草成分产生不良影响的重要靶标。然而，尼古丁对早期生长反应-1（Egr-1）表达的影响在 HSDF 中尚不清楚。
通过 HSDF 中的细胞活力测试评估尼古丁的细胞毒性。通过 Western blot 和实时逆转录-聚合酶链反应评估尼古丁处理后 Egr-1 蛋白和 mRNA 的表达。我们还测量了尼古丁暴露后 HSDF 中 Egr-1 的启动子活性。
暴露于尼古丁的真皮成纤维细胞中 Egr-1 蛋白和 mRNA 水平增加。尼古丁处理刺激了培养的人成纤维细胞中 Egr-1 的启动子活性。
在这项研究中，我们证明了暴露于尼古丁后 HSDF 中的 Egr-1 表达明显增加。
