Mahata S K, Ghosh A
Department of Pharmacology, University of Innsbruck, Austria.
Neuropeptides. 1991 Jan;18(1):29-33. doi: 10.1016/0143-4179(91)90160-k.
Lysine vasopressin was injected (0.25 IU/100 g body wt) intraperitoneally only once to unilaterally splanchnic denervated pigeons. Adrenomedullary catecholamine (CA) content was measured spectrofluorometrically 0.5, 4, 12, 24, 72, 144 and 216 h after administration. The findings revealed that in innervated glands, vasopressin caused 59-74% decrease of norepinephrine (NE) 4 and 24 h after administration while in denervated glands it resulted in 18-65% release of NE 0.5 to 144 h after injection. This indicates that the splanchnic nerve prevents early phase (up to 0.5 h) of vasopressin-induced release of NE. Vasopressin also caused decrease of Epinephrine (E) from both the innervated (68-73%) and denervated (69-74%) glands 0.5 and 4 h after injection indicating that the splanchnic nerve has no effect on vasopressin-induced release of E. Accelerated resynthesis of NE exceeding the control value (44-94%) was encountered 144 and 216 h after injection as compared to a slower increase (14%) in denervated glands 216 h after injection. Higher rate of resynthesis of E was encountered in the innervated glands 12 and 72 h after treatment. The findings clearly point out that the splanchnic nerve accelerates resynthesis of both NE and E induced by vasopressin.
仅对单侧内脏神经去支配的鸽子腹腔注射一次赖氨酸加压素（0.25国际单位/100克体重）。给药后0.5、4、12、24、72、144和216小时，用荧光分光光度法测量肾上腺髓质儿茶酚胺（CA）含量。研究结果显示，在有神经支配的腺体中，加压素在给药后4小时和24小时导致去甲肾上腺素（NE）减少59% - 74%，而在去神经支配的腺体中，注射后0.5小时至144小时导致NE释放18% - 65%。这表明内脏神经可防止加压素诱导的NE释放的早期阶段（长达0.5小时）。加压素在注射后0.5小时和4小时也导致有神经支配（68% - 73%）和去神经支配（69% - 74%）的腺体中的肾上腺素（E）减少，表明内脏神经对加压素诱导的E释放没有影响。与注射后216小时去神经支配腺体中较慢的增加（14%）相比，注射后144小时和216小时遇到NE的再合成加速超过对照值（44% - 94%）。治疗后12小时和72小时，在有神经支配的腺体中遇到较高的E再合成率。研究结果清楚地表明，内脏神经加速了加压素诱导的NE和E的再合成。
