Centre for Neuroscience, Department of Biotechnology, Cochin University of Science and Technology, Cochin, Kerala, India.
Respir Physiol Neurobiol. 2010 Jul 31;172(3):147-53. doi: 10.1016/j.resp.2010.05.009. Epub 2010 May 13.
Molecular processes regulating the cerebellar serotonergic receptors play an important role in the control of respiration and cognitive functions under hypoxia. The present study examined cerebellar 5HT receptor alterations and neuroprotective effect of glucose supplementation prior to current sequence of resuscitation-oxygen and epinephrine supplementation in hypoxic neonatal rats. Hypoxic stress increased the number of total 5HT and 5HT(2A) receptors along with an up-regulation of 5HT transporter and 5HT(2A) receptor gene in cerebellum. These serotonergic alterations were reversed to near control by glucose supplementation. Immunohistochemical studies confirmed the data. Behavioral studies revealed the cognitive impairment due to neonatal hypoxia in the later stages of life and the role of timely glucose supplementation in preventing these behavioral deficits. The enhanced cerebellar 5HT(2A) receptors may act as a modulator of ventilatory response to hypoxia, which can in turn result in cognitive dysfunction. Glucose supplementation helped in managing the serotonergic functional alterations. This has immense clinical significance in neonatal care.
调节小脑 5-羟色胺能受体的分子过程在缺氧状态下对呼吸和认知功能的控制起着重要作用。本研究在缺氧新生大鼠进行复律-氧和肾上腺素补充的序贯治疗之前，观察了小脑 5-HT 受体的改变以及葡萄糖补充的神经保护作用。缺氧应激增加了总 5-HT 和 5-HT（2A）受体的数量，同时上调了小脑内的 5-HT 转运体和 5-HT（2A）受体基因。葡萄糖补充使这些血清素变化接近对照水平。免疫组织化学研究证实了这一数据。行为研究揭示了新生儿缺氧在生命后期导致的认知障碍，以及及时补充葡萄糖在预防这些行为缺陷方面的作用。增强的小脑 5-HT（2A）受体可能作为对缺氧性通气反应的调节剂，这反过来又可能导致认知功能障碍。葡萄糖补充有助于调节血清素功能的改变。这在新生儿护理中具有重要的临床意义。
