Molecular Neurobiology and Cell Biology Unit, Centre for Neuroscience, Department of Biotechnology, Cochin University of Science and Technology, Cochin - 682022, Kerala, India.
Neurosci Lett. 2011 Sep 20;502(3):129-32. doi: 10.1016/j.neulet.2011.05.236. Epub 2011 Jun 12.
Alterations in neurotransmitters and its receptors expression induce brain injury during neonatal hypoxic insult. Molecular processes regulating the serotonergic receptors play an important role in the control of respiration under hypoxic insult. The present study focused on the serotonergic regulation of neonatal hypoxia and its resuscitation methods. Receptor binding assays and gene expression studies were done to evaluate the changes in 5HT(2A) receptors and its transporter in the corpus striatum of hypoxic neonatal rats and hypoxic rats resuscitated with glucose, oxygen and epinephrine. Total 5HT and 5HT(2A) receptor number was increased in hypoxic neonates along with an up regulation of 5HT(2A) receptor and 5HT transporter gene. The enhanced striatal 5HT(2A) receptors modulate the ventilatory response to hypoxia. Immediate glucose resuscitation was found to ameliorate the receptor and transporter alterations. Hypoxia induced ATP depletion mediated reduction in blood glucose levels can be encountered by glucose administration and oxygenation helps in overcoming the anaerobic condition. The adverse effect of immediate oxygenation and epinephrine supplementation was also reported. This has immense clinical significance in establishing a proper resuscitation for the management of neonatal hypoxia.
神经递质及其受体表达的改变会在新生儿缺氧损伤时导致脑损伤。调节 5-羟色胺能受体的分子过程在缺氧损伤下的呼吸控制中起着重要作用。本研究集中于新生儿缺氧及其复苏方法的 5-羟色胺能调节。进行受体结合测定和基因表达研究，以评估缺氧新生大鼠和用葡萄糖、氧气和肾上腺素复苏的缺氧大鼠纹状体中 5-HT(2A)受体及其转运蛋白的变化。在缺氧新生儿中，总 5-HT 和 5-HT(2A)受体数量增加，同时 5-HT(2A)受体和 5-HT 转运体基因上调。增强的纹状体 5-HT(2A)受体调节对缺氧的通气反应。发现立即给予葡萄糖复苏可改善受体和转运体的改变。缺氧诱导的 ATP 耗竭导致血糖水平降低，可通过给予葡萄糖和氧合作用来克服，后者有助于克服无氧条件。也报道了立即给氧和肾上腺素补充的不良影响。这在建立新生儿缺氧管理的适当复苏方面具有重要的临床意义。
