长期高效抗逆转录病毒治疗患者的十二指肠组织中巨噬细胞的 HIV-1 感染。

Macrophage HIV-1 infection in duodenal tissue of patients on long term HAART.

机构信息

Gastroenterology Unit, Hospital Juan A Fernandez, Buenos Aires, Argentina.

出版信息

Antiviral Res. 2010 Aug;87(2):269-71. doi: 10.1016/j.antiviral.2010.05.005. Epub 2010 May 22.

DOI:10.1016/j.antiviral.2010.05.005

PMID:20471997

Abstract

Mucosal surfaces play a major role in human immunodeficiency virus type 1 (HIV-1) transmission and pathogenesis. Since the role of intestinal macrophages as viral reservoirs during chronic HIV-1 infection has not been elucidated, we investigated the effects of successful therapy on intestinal HIV-1 persistence. Intestinal macrophage infection was demonstrated by the expression of p24 antigen by flow cytometry and by the presence of proviral DNA, assessed by PCR. Proviral DNA was detected in duodenal mucosa of HIV-infected patients under treatment with undetectable plasma viral load. These findings confirm that intestinal macrophages can act as viral reservoirs and permit HIV-1 production even after viral suppression following antiretroviral therapy.

摘要

黏膜表面在人类免疫缺陷病毒 1 型（HIV-1）传播和发病机制中起着重要作用。由于在慢性 HIV-1 感染期间肠道巨噬细胞作为病毒储存库的作用尚未阐明，我们研究了成功治疗对肠道 HIV-1 持续存在的影响。通过流式细胞术检测 p24 抗原的表达和通过 PCR 评估前病毒 DNA 的存在来证明肠道巨噬细胞的感染。在接受治疗且血浆病毒载量无法检测到的 HIV 感染患者的十二指肠黏膜中检测到了前病毒 DNA。这些发现证实了肠道巨噬细胞可以作为病毒储存库，并允许 HIV-1 产生，即使在抗逆转录病毒治疗后病毒得到抑制也是如此。