Division of Biomedical Sciences, School of Bio Sciences and Technology, VIT University, Vellore, Tamil Nadu 632 014, India.
Food Chem Toxicol. 2010 Aug-Sep;48(8-9):2133-8. doi: 10.1016/j.fct.2010.05.017. Epub 2010 May 22.
This study was aimed at investigating the phenolic constituents of methanolic and aqueous extracts of Vitex negundo in context of antioxidant potential and inhibition of oxidative stress-induced cytotoxicity. Antioxidant efficacies of both extracts were estimated by their abilities to scavenge DPPH() and to reduce Fe(+3) to Fe(+2). Their protective potential against H(2)O(2)-driven oxidative damage to cells were evaluated by XTT assay in Hep3B (human hepatoma) cells. Total phenolic content of both extracts were estimated, and were tested for correlation with antioxidant potential and protection against oxidative cytotoxicity. Polyphenolic levels in both extracts showed significant (P<0.05) positive correlations with DPPH() scavenging, Fe(+3)-Fe(+2) reduction and percentage decrease in H(2)O(2)-induced cytotoxicity. Major phenolic compounds in the extracts were identified by HPLC. It was concluded that both V. negundo extracts hold considerable potential as antitheses of free radical toxicity by virtue of their polyphenolic constituents, and might have significant clinical roles in prospect.
本研究旨在探讨牡荆甲醇和水提取物中的酚类成分，考察其抗氧化潜力和抑制氧化应激诱导的细胞毒性作用。通过 DPPH(）清除能力和将 Fe(+3)还原为 Fe(+2)的能力来评估两种提取物的抗氧化功效。通过 XTT 法在 Hep3B（人肝癌）细胞中评估它们对 H2O2 驱动的氧化损伤的保护潜力。估算了两种提取物的总酚含量，并测试了它们与抗氧化潜力和对氧化细胞毒性的保护作用之间的相关性。两种提取物中的多酚含量与 DPPH(）清除、Fe(+3)-Fe(+2)还原以及 H2O2 诱导的细胞毒性降低的百分比呈显著（P<0.05）正相关。通过 HPLC 鉴定了提取物中的主要酚类化合物。综上所述，牡荆两种提取物由于其多酚成分，具有相当大的潜力作为自由基毒性的解毒剂，在未来可能具有重要的临床作用。
