Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814, USA.
Vaccine. 2010 Jul 5;28(30):4777-85. doi: 10.1016/j.vaccine.2010.04.099. Epub 2010 May 14.
Previously, we showed that the Shiga toxin type 2 (Stx2)-expressing Escherichia coli O157:H7 strain 86-24 colonized mice better than did its isogenic stx(2) negative mutant. Here, we confirmed that finding by demonstrating that Stx2 given orally to mice increased the levels of the 86-24 stx(2) mutant shed in feces. Then we assessed the impact of Stx2-neutralizing antibodies, administered passively or generated by immunization with an Stx2 toxoid, on E. coli O157:H7 colonization of mice. We found that such antibodies reduced the E. coli O157:H7 burden in infected mice and, as anticipated, also protected them from weight loss and death.
此前，我们表明表达志贺毒素 2 型 (Stx2) 的大肠杆菌 O157:H7 菌株 86-24 比其同工酶 Stx(2) 阴性突变体在小鼠中更好地定植。在这里，我们通过证明口服给予小鼠 Stx2 会增加粪便中 86-24 Stx(2) 突变体脱落的水平，证实了这一发现。然后，我们评估了被动给予或用 Stx2 类毒素免疫产生的 Stx2 中和抗体对小鼠大肠杆菌 O157:H7 定植的影响。我们发现，这些抗体减少了感染小鼠中的大肠杆菌 O157:H7 负担，并且如预期的那样，还保护它们免受体重减轻和死亡的影响。
