Tanaka K I, Atwell R B
Department of Companion Animal Medicine and Surgery, University of Queensland, St Lucia, Australia.
Res Vet Sci. 1991 Jan;50(1):33-7. doi: 10.1016/0034-5288(91)90050-x.
The immunoperoxidase staining characteristics of Dirofilaria immitis and pulmonary tissues from infected dogs were studied by using the following sera: anti-fresh D immitis, anti-processed D immitis, anti-dog IgG, anti-dog IgG Fc, anti-dog IgM and anti-dog C3. Marked staining was observed using anti-fresh D immitis serum. Body cavity fluid and cuticle were strongly stained and hypodermis, muscle, lateral cord, testis, vas deferens, ovary, oviduct and uterus were moderately stained. Oesophagus and intestine were mildly stained. Degenerate worms were stained by all antisera. The intact and cut surfaces of microfilariae and eggs and sperm present in filariae were stained, but not their internal contents. Circulating and stored immotile microfilariae did not stain. Excreted eggs, presumed to be unfertilized and, or, degenerate, stained positively. Immunoperoxidase staining of routinely processed histological samples provides a means of assessing D immitis antigen.
抗新鲜犬恶丝虫血清、抗处理后犬恶丝虫血清、抗犬IgG、抗犬IgG Fc、抗犬IgM和抗犬C3。使用抗新鲜犬恶丝虫血清观察到明显染色。体腔液和角质层被强烈染色，皮下组织、肌肉、侧索、睾丸、输精管、卵巢、输卵管和子宫被中度染色。食管和肠道被轻度染色。退化的虫体被所有抗血清染色。微丝蚴、虫卵和丝虫体内精子的完整表面和切面被染色，但其内部内容物未被染色。循环和储存的无活动能力微丝蚴未被染色。推测为未受精和/或退化的排出虫卵呈阳性染色。常规处理的组织学样本的免疫过氧化物酶染色提供了一种评估犬恶丝虫抗原的方法。
