Hurme M, Serkkola E
Department of Bacteriology and Immunology, University of Helsinki, Finland.
Scand J Immunol. 1991 Jun;33(6):713-8. doi: 10.1111/j.1365-3083.1991.tb02545.x.
The production and mRNA expression of IL-1 alpha and IL-1 beta by human monocytes was examined after two different stimuli, a protein kinase C (PKC) activator phorbol myristate acetate (PMA) and bacterial lipopolysaccharide (LPS). LPS induced production of high levels of both IL-1 alpha and IL-1 beta protein (quantitated with type-specific ELISA assays), while after PMA stimulation only IL-1 beta protein could be detected. The IL-1 alpha and IL-1 beta mRNA levels quantitated by Northern blotting were in line with the respective protein levels and nuclear run off analysis revealed that PMA did not activate the IL-1 alpha transcription. The production of the IL-1 alpha and IL-1 beta protein as well as the mRNA expression could be inhibited with protein kinase inhibitor H7, but not with HA1004, indicating that PKC activation is essential for the activation of these genes. Thus these data indicate that PKC activation alone is sufficient for the induction of the IL-1 beta gene, but some additional signals (provided by LPS) are required for the activation of the IL-1 alpha gene.
在两种不同刺激下，即蛋白激酶C（PKC）激活剂佛波酯肉豆蔻酸酯乙酸酯（PMA）和细菌脂多糖（LPS）刺激后，检测了人单核细胞中白细胞介素-1α（IL-1α）和白细胞介素-1β（IL-1β）的产生及mRNA表达。LPS诱导产生高水平的IL-1α和IL-1β蛋白（通过型特异性ELISA测定法定量），而在PMA刺激后只能检测到IL-1β蛋白。通过Northern印迹法定量的IL-1α和IL-1β mRNA水平与各自的蛋白水平一致，并且细胞核转录分析表明PMA未激活IL-1α转录。IL-1α和IL-1β蛋白的产生以及mRNA表达可以被蛋白激酶抑制剂H7抑制，但不能被HA1004抑制，这表明PKC激活对于这些基因的激活至关重要。因此，这些数据表明单独的PKC激活足以诱导IL-1β基因，但激活IL-1α基因需要一些额外的信号（由LPS提供）。
