Kemp E, Larsen S, Jørgensen K A, Dieperink H, Starklint H
Department of Nephrology, Odense University Hospital, Denmark.
Scand J Urol Nephrol. 1991;25(1):45-9. doi: 10.3109/00365599109024528.
A simple perfusion model was developed to study the events that lead to rejection of renal xenografts. Flush perfusion of the kidneys of 24 rabbits was carried out with blood from rabbits, cats, or humans. Light microscopy showed alterations with neutrophilic granulocytes margination of the vessels after only 15 min of perfusion. In the experiments with human blood the immunofluorescence microscopy showed deposits of immunoglobulins and complement factor III. This simple method is therefore a useful way of studying perfusion in renal xenografts.
开发了一种简单的灌注模型来研究导致肾异种移植排斥反应的事件。用来自兔子、猫或人类的血液对24只兔子的肾脏进行冲洗灌注。光学显微镜显示，灌注仅15分钟后血管就出现了嗜中性粒细胞边缘化改变。在用人血进行的实验中，免疫荧光显微镜显示有免疫球蛋白和补体因子III沉积。因此，这种简单的方法是研究肾异种移植灌注的一种有用方式。
