Center for Biosystematics and Biodiversity, Texas A&M University, College Station, TX 77843-2258, USA.
Anim Genet. 2010 Dec;41(6):630-41. doi: 10.1111/j.1365-2052.2010.02059.x.
Second-generation, sex-specific genetic linkage maps were generated for the economically important estuarine-dependent marine fish Sciaenops ocellatus (red drum). The maps were based on F(1) progeny from each of two single-pair mating families. A total of 237 nuclear-encoded microsatellite markers were mapped to 25 linkage groups. The female map contained 226 markers, with a total length of 1270.9 centiMorgans (cM) and an average inter-marker interval of 6.53 cM; the male map contained 201 markers, with a total length of 1122.9 cM and an average inter-marker interval of 6.03 cM. The overall recombination rate was approximately equal in the two sexes (♀:♂=1.03:1). Recombination rates in a number of linkage intervals, however, differed significantly between the same sex in both families and between sexes within families. The former occurred in 2.4% of mapped intervals, while the latter occurred in 51.2% of mapped intervals. Sex-specific recombination rates varied within chromosomes, with regions of both female-biased and male-biased recombination. Original clones from which the microsatellite markers were generated were compared with genome sequence data for the spotted green puffer, Tetraodon nigroviridis; a total of 43 matches were located in 17 of 21 chromosomes of T. nigroviridis, while seven matches were in unknown portions of the T. nigroviridis genome. The map for red drum provides a new, useful tool for aquaculture, population genetics, and comparative genomics of this economically important marine species.
为经济上重要的依赖河口的海洋鱼类赤点石斑鱼（红鼓鱼）生成了第二代、性别特异性遗传连锁图谱。图谱基于来自两个单对交配家系的每个 F1 后代。总共将 237 个核编码微卫星标记映射到 25 个连锁群上。雌性图谱包含 226 个标记，总长度为 1270.9 厘摩（cM），平均标记间间隔为 6.53 cM；雄性图谱包含 201 个标记，总长度为 1122.9 cM，平均标记间间隔为 6.03 cM。两个性别的总体重组率大致相等（♀：♂=1.03:1）。然而，在两个家系中同一性别和家系内性别之间的许多连锁区间的重组率存在显著差异。前者发生在映射区间的 2.4%，后者发生在映射区间的 51.2%。性特异性重组率在染色体内变化，存在雌性偏和雄性偏重组的区域。生成微卫星标记的原始克隆与斑点绿河豚 Tetraodon nigroviridis 的基因组序列数据进行了比较；总共在 T. nigroviridis 的 21 条染色体中的 17 条上找到了 43 个匹配，而 7 个匹配在 T. nigroviridis 基因组的未知部分。红鼓鱼的图谱为这个经济上重要的海洋物种的水产养殖、群体遗传学和比较基因组学提供了一个新的、有用的工具。
