Fogh J, Loveless J
J Natl Cancer Inst. 1978 Apr;60(4):895-8.
Sixteen established cell lines of simian virus 40 (SV40)-transformed human amnion cells were examined for SV40 production. Many of these lines produced SV40 for extensive periods. Virus production had not ceased for 2 lines after 18 months, for 3 lines after 12 months, and for 3 lines at 3 months after recovery from "crisis". Three lines became virus-free in the first month, 1 line in the second month, 1 in the third month, and 1 in the fourth month, and 2 lines stopped virus production between 6 and 11 months after recovery. The virus titers were relatively low. Inclusion body-containing cells were infrequent. In contrast, in most cultures of SV40-transformed human fibroblasts rescued from crisis, no infectious virus was demonstrated, although exceptions have been reported. Virus was produced after heterokaryon formation of cells of the virus-free amnion lines with CV-1 cells in the presence of inactivated Sendai virus, as observed for SV40-transformed human fibroblasts. During the crisis period, some of the SV40-transformed amnion cells produced substantial amounts of virus. Titers decreased during the later periods of crisis. The most pronounced decrease in titers was in cultures from which established lines were recovered.
对16株已建立的猿猴病毒40（SV40）转化的人羊膜细胞系进行了SV40产生情况的检测。这些细胞系中的许多在很长一段时间内都能产生SV40。18个月后仍有2株细胞系未停止产生病毒，12个月后有3株，从“危机”中恢复3个月后有3株。3株在第一个月就不再产生病毒，1株在第二个月，1株在第三个月，1株在第四个月，还有2株在恢复后6至11个月之间停止产生病毒。病毒滴度相对较低。含包涵体的细胞很少见。相比之下，在大多数从危机中挽救回来的SV40转化的人成纤维细胞培养物中，虽然有例外报道，但未检测到感染性病毒。如在SV40转化的人成纤维细胞中所观察到的，在无病毒羊膜细胞系的细胞与CV - 1细胞在灭活仙台病毒存在的情况下形成异核体后产生了病毒。在危机期间，一些SV40转化的羊膜细胞产生了大量病毒。在危机后期滴度下降。滴度下降最明显的是那些从中恢复建立细胞系的培养物。
