Plant Virology Unit, Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi 110012, India.
Arch Virol. 2010 Aug;155(8):1361-5. doi: 10.1007/s00705-010-0687-z. Epub 2010 May 18.
Watermelon bud necrosis virus (WBNV), a member of the genus Tospovirus, family Bunyaviridae is an important viral pathogen in watermelon cultivation in India. The complete genome sequence properties of WBNV are not available. In the present study, the complete M RNA sequence and the genome organisation of a WBNV isolate infecting watermelon in Delhi (WBNV-wDel) were determined. The M RNA was 4,794 nucleotides (nt) long and potentially coded for a movement protein (NSm) of 34.22 kDa (307 amino acids) on the viral sense strand and a Gn/Gc glycoprotein precursor of 127.15 kDa (1,121 amino acids) on the complementary strand. The two open reading frames were separated by an intergenic region of 402 nt. The 5' and 3' untranslated regions were 55 and 47 nt long, respectively, containing complementary termini typical of tospoviruses. WBNV-wDel was most closely related (79.1% identity) to Groundnut bud necrosis virus, an important tospovirus that occurs in several crops in India, and was different (63.3-75.2% identity) from the other cucurbit-infecting tospoviruses known to occur in Taiwan and Japan. Sequence analysis of NSm and Gn/Gc revealed phylogenetic incongruence between WBNV-wDel and another isolate originating from central India (WBNV-Wm-Som isolate). The Wm-Som isolate showed evolutionary divergence from the wDel isolate in the Gn/Gc protein (74.6% identity) potentially due to recombination with the other tospoviruses that are known to occur in India. This is the first report of a comparison of complete sequences of M RNA of WBNV.
西瓜坏死斑点病毒（WBNV），是布尼亚病毒科 Bunyaviridae 病毒属的一种成员，是印度西瓜种植中的一种重要病毒病原体。目前，尚未获得 WBNV 的完整基因组序列特性。在本研究中，确定了感染德里西瓜的 WBNV 分离株（WBNV-wDel）的完整 M RNA 序列和基因组结构。M RNA 长 4794 个核苷酸（nt），在病毒正义链上可能编码一个 34.22 kDa（307 个氨基酸）的运动蛋白（NSm），在互补链上编码一个 127.15 kDa（1121 个氨基酸）的 Gn/Gc 糖蛋白前体。两个开放阅读框由 402 个核苷酸的基因间区隔开。5'和 3'非翻译区分别长 55 和 47 个核苷酸，含有典型的 Tospovirus 互补末端。WBNV-wDel 与在印度几种作物中发生的重要 Tospovirus——花生坏死斑点病毒最为密切相关（79.1%的同一性），与在台湾和日本发生的其他侵染葫芦科的 Tospovirus 不同（63.3-75.2%的同一性）。NSm 和 Gn/Gc 的序列分析表明，WBNV-wDel 与来自印度中部的另一个分离株（WBNV-Wm-Som 分离株）之间存在系统发育不一致。Wm-Som 分离株在 Gn/Gc 蛋白中与 wDel 分离株发生进化分歧（74.6%的同一性），可能是由于与已知在印度发生的其他 Tospovirus 重组所致。这是首次报道 WBNV M RNA 完整序列的比较。
