Mikrobiologie, Fachbereich für Biologie, Universität Freiburg, Schänzle Strasse 1, 79104 Freiburg, Germany.
Arch Microbiol. 2010 Jul;192(7):549-57. doi: 10.1007/s00203-010-0583-7. Epub 2010 May 18.
CinA is a conserved bacterial protein that has been reported to play an important role during competence in Streptococcus pneumoniae by recruiting the RecA protein to the cell membrane. Here, we provide information on the homologous CinA in Bacillus subtilis. We found that the synthesis of CinA is upregulated during stationary phase in all cells. The loss of CinA has a mild effect during competence, but it has no influence on the localization of RecA. CinA was observed to be associated with the nucleoid in the cell, and not with the cell membrane, as shown for S. pneumoniae. Purified CinA is a soluble protein, probably forming trimers, like other homologues, which share a domain with CinA that has been reported to be involved in molybdopterine biosynthesis. Our results suggest that CinA plays a nucleoid-associated general role in cells entering stationary phase that is not specific to competence in B. subtilis and possibly in many other bacteria.
CinA 是一种保守的细菌蛋白，据报道，它在肺炎链球菌的感受态中通过将 RecA 蛋白招募到细胞膜上发挥重要作用。在这里，我们提供了枯草芽孢杆菌中同源 CinA 的信息。我们发现 CinA 的合成在所有细胞的静止期都被上调。 CinA 的缺失在感受态中仅有轻微的影响，但对 RecA 的定位没有影响。与肺炎链球菌不同，观察到 CinA 与细胞的核体相关联，而不是与细胞膜相关联。纯化的 CinA 是一种可溶性蛋白，可能像其他同源物一样形成三聚体，它们共享一个与 CinA 报告的参与钼蝶呤生物合成的结构域。我们的结果表明，CinA 在进入静止期的细胞中发挥核体相关的一般作用，这种作用不仅限于枯草芽孢杆菌的感受态，也可能存在于许多其他细菌中。
