Department of Pathology, University of Cambridge, Cambridge, UK.
Eur J Immunol. 2010 Aug;40(8):2221-9. doi: 10.1002/eji.200939998.
The immunomodulatory effect of Schistosoma mansoni antigens has often been attributed to interaction with PRR expressed on APC. Our previous work has shown that S. mansoni-soluble egg antigen (SEA) can induce, together with a Th2 response, TGF-β-dependent Foxp3 expression in naïve CD4(+) T cells from NOD mice. We found that SEA can directly upregulate the expression of surface-bound TGF-β in purified CD4(+) T cells in the absence of accessory cell interactions. In this study, we show that the C-type lectin receptors DEC-205 and galectin-3 were involved in the direct interaction between S. mansoni antigens and CD4(+) T cells. SEA was able to enhance CD4(+) T-cell secretion of bioactive TGF-β in response to TLR2 ligand stimulation, in the absence of APC. We also show that TLR2 expressed on CD4(+) T cells was important for the Foxp3 expression induced by SEA.
曼氏血吸虫抗原的免疫调节作用通常归因于与 APC 上表达的 PRR 的相互作用。我们之前的工作表明，曼氏血吸虫可溶性虫卵抗原 (SEA) 可以与 NOD 小鼠的幼稚 CD4(+) T 细胞中的 Th2 反应一起诱导 TGF-β 依赖性 Foxp3 表达。我们发现 SEA 可以在没有辅助细胞相互作用的情况下直接上调纯化的 CD4(+) T 细胞表面结合的 TGF-β 的表达。在这项研究中，我们表明 C 型凝集素受体 DEC-205 和半乳糖凝集素-3 参与了曼氏血吸虫抗原与 CD4(+) T 细胞之间的直接相互作用。SEA 能够增强 CD4(+) T 细胞对 TLR2 配体刺激的生物活性 TGF-β 的分泌，而无需 APC。我们还表明，TLR2 在 CD4(+) T 细胞上的表达对于 SEA 诱导的 Foxp3 表达很重要。
