Yang Di, Qiu Li-hong, Li Ren, Li Zi-mu, Li Chen
Dept. of Endodontics, School of Stomatology, China Medical University, Shenyang 110002, China.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2010 Apr;28(2):135-8.
To quantify the interleukin (IL)-1beta mRNA and IL-6 mRNA expression induced by lipopolysaccharides ([PS) extracted from Porphyromonoas endodontalis (P. endodontalis) in osteoblasts, and to relate P. endodontalis LPS to the bone resorptive pathogenesis in the lesions of chronic apical periodontitis.
MG63 cells was pretreated with PD98059 or SB203580 for 1 h and then treated with P. endodontolis LPS for 6 h. The expression of IL-1beta mRNA and IL-6 mRNA were detected by reverse transcription polymerase chain reaction (RT-PCR) technique.
The production of IL-1beta mRNA induced by P. endodontalis LPS decreased in osteoblasts pretreated with PD98059. Both of the production of IL-1beta mRNA and JL-6 mRNA induced by P. endodontalis LPS decreased in osteoblasts pretreated with SB203580.
The synthesis of IL-1beta mRNA stimulated by Pendodontalis LPS in MG63 probably occur via extracellular signal-regulated kinase (ERK) 1/2 and p38 mitogen activated protein kinase (MAPK) signal transduction system. The synthesis of IL-6 mRNA stimulated by P.endodontalis LPS in MG63 probahly occur via p38MAPK signal transduction system.
定量检测牙髓卟啉单胞菌（牙髓卟啉菌）提取的脂多糖（LPS）诱导成骨细胞中白细胞介素（IL）-1β mRNA和IL-6 mRNA的表达，并探讨牙髓卟啉菌LPS与慢性根尖周炎病变中骨吸收发病机制的关系。
MG63细胞先用PD98059或SB203580预处理1小时，然后用牙髓卟啉菌LPS处理6小时。采用逆转录聚合酶链反应（RT-PCR）技术检测IL-1β mRNA和IL-6 mRNA的表达。
用PD98059预处理的成骨细胞中，牙髓卟啉菌LPS诱导的IL-1β mRNA产生减少。用SB203580预处理的成骨细胞中，牙髓卟啉菌LPS诱导的IL-1β mRNA和IL-6 mRNA产生均减少。
牙髓卟啉菌LPS刺激MG63中IL-1β mRNA的合成可能通过细胞外信号调节激酶（ERK）1/2和p38丝裂原活化蛋白激酶（MAPK）信号转导系统发生。牙髓卟啉菌LPS刺激MG63中IL-6 mRNA的合成可能通过p38MAPK信号转导系统发生。
