Mastromei G, Barberio C, Pistolesi S, Delfino G
Dipartimento di Biologia animale e Genetica, Universitá degli studi di Firenze, Italy.
Toxicon. 1991;29(3):321-8. doi: 10.1016/0041-0101(91)90285-y.
The skin venom of the yellow bellied toad Bombina variegata pachypus has an antimicrobial activity which seems to be correlated to the presence of a 6700 mol. wt polypeptide. This polypeptide was purified by electroelution from SDS-urea-polyacrylamide gels and characterized for its antimicrobial activity. A bactericidal action was detected at concentrations with little or no cytolytic effect. The determination of the Minimal Inhibitory Concentration showed that there was activity against gram positive and gram negative bacteria and also against yeasts. The skin secretions of three other anuran species (Bufo viridis, Hyla arborea and Discoglossus pictus) were examined for the presence of antimicrobial activities. Only the Hyla arborea secretion exhibited antimicrobial properties. A small amount of a 6700 mol. wt polypeptide was detected among the Hyla secreted products.
黄腹蟾蜍（Bombina variegata pachypus）皮肤毒液具有抗菌活性，这似乎与一种分子量为6700道尔顿的多肽的存在有关。该多肽通过从SDS-尿素-聚丙烯酰胺凝胶中电洗脱进行纯化，并对其抗菌活性进行了表征。在几乎没有或没有细胞溶解作用的浓度下检测到杀菌作用。最低抑菌浓度的测定表明，它对革兰氏阳性菌和革兰氏阴性菌以及酵母菌都有活性。对其他三种无尾目物种（绿蟾蜍、树蛙和饰纹姬蛙）的皮肤分泌物进行了抗菌活性检测。只有树蛙的分泌物表现出抗菌特性。在树蛙的分泌产物中检测到少量分子量为6700道尔顿的多肽。
