纤溶毒素亚基与抗纤溶毒素及某些蛇毒的兔抗血清之间的免疫学关系。

Immunological relationships between the subunits of textilotoxin and rabbit antisera raised against textilotoxin and some snake venoms.

作者信息

Pearson J A, Tyler M I, Howden M E

机构信息

Department of Biological Sciences, Deakin University, Geelong, Victoria, Australia.

出版信息

Toxicon. 1991;29(3):375-8. doi: 10.1016/0041-0101(91)90291-x.

DOI:10.1016/0041-0101(91)90291-x

PMID:2048151

Abstract

The binding of textilotoxin and its subunits A, B, C and D to polyclonal rabbit antisera directed against textilotoxin, Australian common brown snake (Pseudonaja textilis) and tiger snake (Notechis scutatus scutatus) venoms was studied by ELISA. Subunit D showed greatest reactivity with antisera to textilotoxin and brown snake venom. The phospholipase A2 active subunit A reacted more strongly with antisera to tiger snake venom in keeping with the high degree of homology between the amino acid sequences of subunit A and notexin from tiger snake venom. Subunit A was the only subunit lethal to mice, but at doses 1000-fold greater than for textilotoxin.

摘要

通过酶联免疫吸附测定法（ELISA）研究了纤蛇毒素及其亚基A、B、C和D与针对纤蛇毒素、澳大利亚普通棕蛇（杜氏眼镜蛇）和虎蛇（盾鼻虎蛇）毒液的兔多克隆抗血清的结合情况。亚基D与针对纤蛇毒素和棕蛇毒液的抗血清反应最强。具有磷脂酶A2活性的亚基A与针对虎蛇毒液的抗血清反应更强，这与亚基A的氨基酸序列与虎蛇毒液中的虎蛇毒素高度同源一致。亚基A是唯一对小鼠致死的亚基，但所需剂量比纤蛇毒素大1000倍。