Pearson J A, Tyler M I, Howden M E
Department of Biological Sciences, Deakin University, Geelong, Victoria, Australia.
Toxicon. 1991;29(3):375-8. doi: 10.1016/0041-0101(91)90291-x.
The binding of textilotoxin and its subunits A, B, C and D to polyclonal rabbit antisera directed against textilotoxin, Australian common brown snake (Pseudonaja textilis) and tiger snake (Notechis scutatus scutatus) venoms was studied by ELISA. Subunit D showed greatest reactivity with antisera to textilotoxin and brown snake venom. The phospholipase A2 active subunit A reacted more strongly with antisera to tiger snake venom in keeping with the high degree of homology between the amino acid sequences of subunit A and notexin from tiger snake venom. Subunit A was the only subunit lethal to mice, but at doses 1000-fold greater than for textilotoxin.
通过酶联免疫吸附测定法（ELISA）研究了纤蛇毒素及其亚基A、B、C和D与针对纤蛇毒素、澳大利亚普通棕蛇（杜氏眼镜蛇）和虎蛇（盾鼻虎蛇）毒液的兔多克隆抗血清的结合情况。亚基D与针对纤蛇毒素和棕蛇毒液的抗血清反应最强。具有磷脂酶A2活性的亚基A与针对虎蛇毒液的抗血清反应更强，这与亚基A的氨基酸序列与虎蛇毒液中的虎蛇毒素高度同源一致。亚基A是唯一对小鼠致死的亚基，但所需剂量比纤蛇毒素大1000倍。
