Systems Biophysics, Physics Department, Center for Nanoscience, Ludwig Maximilians Universität München, Amalienstrasse 54, 80799 München, Germany.
Phys Rev Lett. 2010 May 7;104(18):188102. doi: 10.1103/PhysRevLett.104.188102.
The hallmark of living matter is the replication of genetic molecules and their active storage against diffusion. We implement both in the simple nonequilibrium environment of a temperature gradient. Convective flow both drives the DNA replicating polymerase chain reaction while concurrent thermophoresis accumulates the replicated 143 base pair DNA in bulk solution. The time constant for accumulation is 92 s while DNA is doubled every 50 s. The experiments explore conditions in pores of hydrothermal rock which can serve as a model environment for the origin of life.
生命物质的标志是遗传分子的复制及其对扩散的主动存储。我们在温度梯度的简单非平衡环境中实现了这两者。对流流既驱动了复制 DNA 的聚合酶链反应，同时热泳又将复制的 143 个碱基对 DNA 在大量溶液中积累。积累的时间常数为 92 秒，而 DNA 每 50 秒复制一次。这些实验探索了热液岩石孔隙中的条件，这些条件可以作为生命起源的模型环境。
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