Environmental Microbiology Section, Indian Institute of Toxicology Research (CSIR), M.G. Marg, Lucknow 226001, Uttar Pradesh, India.
J Hazard Mater. 2010 Aug 15;180(1-3):730-4. doi: 10.1016/j.jhazmat.2010.04.100. Epub 2010 May 18.
Present study deals the effect of bacteria treated and untreated post-methanated distillery effluent (PMDE) on germination, seedling growth and amylase activity in Phaseolus mungo L. seeds. Results revealed that untreated PMDE was highly toxic in nature carrying high BOD, COD values along with high metals content. But, after bacterial treatment, these values were reduced by 64.58 and 74.20%, respectively. It was observed that 40% untreated PMDE has no inhibitory effect on seed germination but 60, 80 and 100% PMDE has inhibited 20, 40 and 60% germination, respectively while 100% germination was recorded up to 60% treated PMDE. Moreover, 40 and 60% PMDE has shown deleterious effects on seedling growth parameter and seeds treated with 80 and 100% PMDE showed no root development. However, 20% bacteria treated PMDE was found most suitable for plant growth possibly due to presence of optimum level of nutrients. Further, Phaseolus seeds treated with 60 and 80% untreated PMDE showed reduced amylase activity and no amylase activity was observed in seeds treated with 100% untreated PMDE. But, seeds treated with bacterial degraded PMDE showed amylase activity and molecular weight of alpha-amylase enzyme determined by SDS-PAGE was approximately 47.5, 46 and 44.5 kDa, respectively.
本研究探讨了未经处理和经细菌处理后的沼气发酵后废水（PMDE）对绿豆种子萌发、幼苗生长和淀粉酶活性的影响。结果表明，未经处理的 PMDE 具有很高的毒性，BOD 和 COD 值都很高，同时还含有大量金属。但是，经过细菌处理后，这些值分别降低了 64.58%和 74.20%。结果发现，40%未经处理的 PMDE 对种子萌发没有抑制作用，但 60%、80%和 100%的 PMDE 分别抑制了 20%、40%和 60%的萌发，而处理后的 PMDE 达到 100%时，种子萌发率为 60%。此外，40%和 60%的 PMDE 对幼苗生长参数有有害影响，用 80%和 100%的 PMDE 处理的种子没有根的发育。然而，20%经细菌处理的 PMDE 最适合植物生长，这可能是由于存在最佳水平的养分。此外，用 60%和 80%未经处理的 PMDE 处理的绿豆种子的淀粉酶活性降低，用 100%未经处理的 PMDE 处理的种子没有淀粉酶活性。但是，用细菌降解的 PMDE 处理的种子显示出淀粉酶活性，并且通过 SDS-PAGE 测定的α-淀粉酶酶的分子量约为 47.5、46 和 44.5 kDa。
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