Department of Pharmaceutical Biology, GUIDE, University of Groningen, 9713 AV Groningen, The Netherlands.
Plant Cell Physiol. 2010 Jul;51(7):1219-28. doi: 10.1093/pcp/pcq073. Epub 2010 May 18.
From Artemisia annua L., a new oxidoreductase (Red 1) was cloned, sequenced and functionally characterized. Through bioinformatics, heterologous protein expression and enzyme substrate conversion assays, the elucidation of the enzymatic capacities of Red1 was achieved. Red1 acts on monoterpenoids, and in particular functions as a menthone:neomenthol oxidoreductase. The kinetic parameter k(cat)/K(m) was determined to be 939-fold more efficient for the reduction of (-)-menthone to (+)-neomenthol than results previously reported for the menthone:neomenthol reductase from Mentha x piperita. Based on its kinetic properties, the possible use of Red1 in biological crop protection is discussed.
从青蒿（Artemisia annua L.）中克隆、测序并功能表征了一种新的氧化还原酶（Red 1）。通过生物信息学、异源蛋白表达和酶底物转化实验，阐明了 Red1 的酶学特性。Red1 作用于单萜类化合物，特别是作为薄荷酮：新薄荷醇氧化还原酶发挥作用。确定 (-)-薄荷酮还原为 (+)-新薄荷醇的 k(cat)/K(m)动力学参数比以前报道的薄荷醇：新薄荷醇还原酶（来自薄荷 Mentha x piperita）的结果高 939 倍。基于其动力学特性，讨论了 Red1 在生物作物保护中的可能用途。
