Currie Geoffrey, Clarke Simon, Rogiers Suzy, Wheat Janelle
Faculty of Science, Charles Sturt University, Wagga Wagga, New South Wales, Australia.
J Nucl Med Technol. 2010 Jun;38(2):76-80. doi: 10.2967/jnmt.109.071886. Epub 2010 May 18.
The accumulation of technetium by plants has been widely studied and reported in the literature from the perspective of the incorporation of environmental (99)Tc into the food chain. Pertechnetate (TcO(4)(-)) is the most stable surface chemical form of technetium and is known to be extracted by plant roots, transported by the xylem, and reduced in the leaves; however, the mechanism of action is not entirely clear. Measuring the distribution of technetium in plants has been challenging, many questions remaining unanswered. To date, tracer studies for plant physiology (radionuclide and color dye) have relied on destructive sampling, prohibiting repeat-design experimentation. This article explores the technical issues relating to the application of scintigraphic imaging to plant physiology. The benefits and limitations of methods for introducing radiotracers to plants are outlined. Strategies for the successful labeling of various plant organs with (99m)Tc and several unanticipated artifacts are described. The relevance of these labeling experiments to the study of plant vascular transport is explained, and strategies for optimizing the scintigraphic imaging of plants are outlined. Assessing plant physiology is an emerging frontier, especially given the growing importance of water management and the increased competing demand for crops as biofuels.
从环境中（99）Tc进入食物链的角度来看，植物对锝的积累已在文献中得到广泛研究和报道。高锝酸盐（TcO(4)(-)）是锝最稳定的表面化学形态，已知它可被植物根系吸收，通过木质部运输，并在叶片中被还原；然而，其作用机制尚不完全清楚。测量植物中锝的分布一直具有挑战性，许多问题仍未得到解答。迄今为止，用于植物生理学的示踪研究（放射性核素和彩色染料）依赖于破坏性取样，这使得重复设计实验变得不可能。本文探讨了与闪烁成像在植物生理学中的应用相关的技术问题。概述了将放射性示踪剂引入植物的方法的优缺点。描述了用（99m）Tc成功标记各种植物器官的策略以及一些意外的伪影。解释了这些标记实验与植物维管运输研究的相关性，并概述了优化植物闪烁成像的策略。评估植物生理学是一个新兴领域，特别是考虑到水资源管理的重要性日益增加以及对作为生物燃料的作物的竞争需求不断增加。
