Hospital de Clínicas de Porto Alegre, and Department of Internal Medicine, Federal University of Rio Grande do Sul, Rua Ramiro Barcelos 2350, Prédio 12, andar 4, 90035-003 Porto Alegre, RS, Brazil.
J Clin Endocrinol Metab. 2010 Aug;95(8):3909-17. doi: 10.1210/jc.2009-2674. Epub 2010 May 19.
The Ala54Thr polymorphism of FABP2 gene increases affinity of intestinal fatty acid-binding protein 2 for long-chain dietary fatty acids (FA) in subjects without diabetes.
Our objective was to evaluate whether the Ala54Thr polymorphism of the FABP2 gene influences the FA composition in chylomicrons after a standard meal in patients with type 2 diabetes.
This clinical trial studied 11 patients with TT and 15 patients with AA genotypes for Ala54Thr polymorphism of FABP2 gene selected from a Brazilian type 2 diabetic cohort. FA in chylomicrons (gas chromatography), plasma glucose, and serum triglycerides were measured after an overnight fast at baseline and, after a standard test meal, at 2-h intervals during 8 h.
During the test meal, the curves response of unsaturated FA of patients with TT genotype were different from patients with AA genotype: only patients with TT genotype exhibited an increase, with a postprandial peak at 6 h in monounsaturated FA [0.479 (0.248-0.709) to 1.674 (0.698-2.650) g/liter], polyunsaturated FA [0.338 (0.154-0.522) to 1.827 (0.389-3.265) g/liter], and trans-unsaturated FA [0.025 (0.013-0.037) to 0.122 (0.040-0.205) g/liter] (generalized estimating equations for repeated measurements: P<0.05 for all). The increase of saturated FA did not reach statistical significance. Diabetes treatment, previous diet, FA at baseline, and the increase of plasma glucose and triglycerides during the test meal were not different between TT and AA genotypes.
The TT genotype in Ala54Thr polymorphism of FABP2 gene in patients with type 2 diabetes increased dietary FA absorption, and this might increase the susceptibility to the effects of dietary lipids.
FABP2 基因的 Ala54Thr 多态性增加了肠道脂肪酸结合蛋白 2 对无糖尿病受试者中长链膳食脂肪酸 (FA) 的亲和力。
我们的目的是评估 2 型糖尿病患者中 FABP2 基因的 Ala54Thr 多态性是否影响标准餐后乳糜微粒中的 FA 组成。
本临床试验研究了从巴西 2 型糖尿病队列中选择的 Ala54Thr 多态性 FABP2 基因 TT 和 AA 基因型的 11 例患者和 15 例患者。在禁食过夜的基础上和 8 小时内每 2 小时进行 2 小时的标准测试餐后，测量乳糜微粒中的 FA（气相色谱法）、血糖和血清甘油三酯。
在测试餐期间，TT 基因型患者的不饱和 FA 曲线反应与 AA 基因型患者不同：只有 TT 基因型的患者表现出增加，在 6 小时时出现单不饱和 FA 的餐后高峰[0.479（0.248-0.709）至 1.674（0.698-2.650）g/L]、多不饱和 FA [0.338（0.154-0.522）至 1.827（0.389-3.265）g/L]和反式不饱和 FA [0.025（0.013-0.037）至 0.122（0.040-0.205）g/L]（重复测量的广义估计方程：所有 P<0.05）。饱和 FA 的增加未达到统计学意义。TT 和 AA 基因型之间的糖尿病治疗、既往饮食、基线 FA 以及测试餐期间血糖和甘油三酯的增加没有差异。
2 型糖尿病患者 FABP2 基因 Ala54Thr 多态性的 TT 基因型增加了膳食 FA 的吸收，这可能增加了对膳食脂质影响的易感性。
