免疫缺陷大鼠肺炎的病原体卡氏肺孢子虫的诊断方法。

A diagnostic method for pneumocystis carinii a causative agent of pneumonia in immunodeficient rats.

机构信息

Institute of Experimental Animal Sciences, Osaka University Graduate School of Medicine, Suita, Osaka, Japan.

出版信息

Exp Anim. 2010;59(2):261-7. doi: 10.1538/expanim.59.261.

DOI:10.1538/expanim.59.261

PMID:20484862

Abstract

Immunodeficient animals are in demand in current biomedical research, and they contribute to medical progress. In Pneumocystis infections, a specific histological diagnostic tool may be immunochemistry (IC). However, it was recently reported that the antibody (3F6) was not suitable for detecting Pneumocystis in rats. We purchased another antibody [PNC007] from a commercial source for IC. We could detect positive signals at identical locations with IC and Toluidine blue O in lungs of infected rats. These results corresponded to the results obtained with PCR. We should study the relationship between unexpected positive signals seen in IC and trophic forms in lungs of infected rats. We could clinically diagnose pneumonia caused by Pneumocystis carinii with the diagnostic method we developed.

摘要

免疫缺陷动物在当前的生物医学研究中需求量很大，它们为医学进步做出了贡献。在卡氏肺孢子虫感染中，一种特定的组织学诊断工具可能是免疫化学（IC）。然而，最近有报道称，抗体（3F6）不适合检测大鼠中的卡氏肺孢子虫。我们从商业来源购买了另一种用于 IC 的抗体 [PNC007]。我们可以在感染大鼠的肺中用 IC 和甲苯胺蓝 O 检测到相同位置的阳性信号。这些结果与 PCR 获得的结果相对应。我们应该研究在 IC 中观察到的意外阳性信号与感染大鼠肺中的营养形式之间的关系。我们可以用我们开发的诊断方法对由卡氏肺孢子虫引起的肺炎进行临床诊断。