[墨西哥锡那罗亚皮肤利什曼病患者血清对墨西哥利什曼原虫（利什曼原虫属）抗原的反应]
[Reaction of Leishmania (Leishmania) mexicana antigens by sera of patients with cutaneous leishmaniasis from Sinaloa, Mexico].
作者信息
Salazar-Mejía Patricia Guadalupe, Tejeda-Aguirre Celia Rosa, López-Moreno Héctor Samuel
机构信息
Laboratorio de Biomedicina, Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Sinaloa, Sinaloa, México.
出版信息
Salud Publica Mex. 2010 Mar-Apr;52(2):165-9. doi: 10.1590/s0036-36342010000200009.
OBJECTIVE
To detect Leishmania mexicana antigens reacting with sera of patients with cutaneous leishmaniasis (CL).
MATERIAL AND METHODS
A crude extract of L. mexicana was used as antigen for 2-D Western blot using sera from 5 patients with CL and controls from Sinaloa, Mexico during 2008.
RESULTS
Five antigens were detected in the five infected patients analyzed; their molecular weights and isoelectric points were: 26 kDa (pI 7.8), 27 kDa (pI 8.1), 28 kDa (pI 8.6), 29 kDa (pI 8.5) and 31 kDa (pI 9.0).
CONCLUSION
New potentially immunodominant L. mexicana antigens were detected, suggesting that this parasite could be the species responsible for human infection in Sinaloa.
目的
检测与皮肤利什曼病（CL）患者血清发生反应的墨西哥利什曼原虫抗原。
材料与方法
2008年，使用墨西哥利什曼原虫的粗提物作为抗原，对来自墨西哥锡那罗亚州的5例CL患者血清和对照进行二维蛋白质印迹分析。
结果
在所分析的5例感染患者中检测到5种抗原；它们的分子量和等电点分别为：26 kDa（pI 7.8）、27 kDa（pI 8.1）、28 kDa（pI 8.6）、29 kDa（pI 8.5）和31 kDa（pI 9.0）。
结论
检测到新的潜在免疫显性墨西哥利什曼原虫抗原，提示该寄生虫可能是锡那罗亚州人类感染的病原体。
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