Faculty of Chemical Engineering, Department of Chemistry, Aristotle University of Thessaloniki, Thessaloniki, Greece.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2010;45(8):1017-25. doi: 10.1080/10934521003772444.
Biological methods of wastewater treatment have been proved very effective for bioremediation of polluted sites. In this study, the degrading abilities of two bacteria Pseudomonas sp. and Staphylococcus xylosus, towards 1,2-dichlorobenzene (1,2-DCB), 2,4-dichlorophenol (2,4-DCP) and 4-Cl-m-cresol, are compared. Culture history and the presence of glucose as carbon source have been used for the optimization of cell's performance. 1,2-DCB showed the higher values of effective concentration (EC(50)), 1.04 and 0.84 mM with Pseudomonas sp. and S. xylosus respectively, whereas no substrate-inhibition appeared, in contrary to 4-Cl-m-cresol, that was more persistent in biodegradation by both bacteria. 2,4-DCP was less assimilated compared to 1,2-DCB, whereas bacterial specificity was higher, as it was found by the estimation of the half-saturation constant of 0.36 and 0.26 mM with Pseudomonas sp. and S. xylosus, respectively.
生物法在受污染场地的生物修复中已被证明非常有效。在这项研究中，比较了两种细菌假单胞菌和葡萄球菌对 1,2-二氯苯（1,2-DCB）、2,4-二氯苯酚（2,4-DCP）和 4-Cl-m-甲酚的降解能力。通过培养历史和葡萄糖作为碳源的存在，优化了细胞的性能。假单胞菌和 S. xylosus 对 1,2-DCB 的有效浓度（EC（50））分别为 1.04 和 0.84mM，而 4-Cl-m-甲酚则没有出现基质抑制，与相反，4-Cl-m-甲酚在两种细菌的生物降解中更持久。2,4-DCP 的同化率低于 1,2-DCB，而细菌的特异性更高，假单胞菌和 S. xylosus 的半饱和常数估计值分别为 0.36 和 0.26mM。
