Tarnowska-Madra Urszula, Leibschang Jerzy, Kowalska Bozena, Filipp Ewa, Kozar Agata, Nimer Anna, Maciejewski Tomasz
Klinika Poloznictwa I Ginekologii Instytutu Matki i Dziecka w Warszawie.
Ginekol Pol. 2010 Mar;81(3):192-6.
The purpose of the study was to compare concentrations of inflammatory and Th1/Th2 cytokines in serum obtained from women with preeclampsia or severe pregnancy hypertension versus normotensive controls.
The study group consisted of 34 pregnant women with hypertension over 140/90mmHg and proteinuria over 0.3 g/day or severe pregnancy hypertension. 16 healthy pregnant women comprised the control group. The concentration of IL-2, IL-4, IL-6, IL-10, TNFalpha and IFNgamma was measured with Cytometric Bead Array Human Th1/Th2 Cytokine Kit II (Becton Dickinson). U-Mann Whitney test was used for the comparison of the results.
We found statistically significantly increased concentrations of IFNgamma: 8.4 +/- 5.3 pg/ml vs. 4.2 +/- 3.2 pg/ml (p = 0.02), TNFalpha: 1.5 +/- 0.7 pg/ml vs. 0.7 +/- 0.3 pg/ml (p = 0.04) and IL-2: 1.3-0.6 pg/ml vs. 0.6 +/- 0.4 pg/ml (p = 0.01) in the studied group. The level of IL-6 35.5 +/- 21.0 pg/ml vs. 19.8 +/- 12.3 pg/ml was also increased but the difference did not reach statistical significance. Concentrations of IL-4 and IL- 10 were similar in both groups.
Increased concentrations of Th1 cytokines (IFNgamma, IL-2) in the serum of women with preeclampsia suggests an exaggerated cytotoxic activity of blood in this pathology accompanied by an increase in the levels of inflammatory cytokines TNFalpha and IL-6.
本研究旨在比较先兆子痫或重度妊娠高血压妇女与血压正常对照组妇女血清中炎症细胞因子和Th1/Th2细胞因子的浓度。
研究组由34名血压超过140/90mmHg且蛋白尿超过0.3g/天的高血压孕妇或重度妊娠高血压孕妇组成。16名健康孕妇组成对照组。使用细胞计数珠阵列人Th1/Th2细胞因子检测试剂盒II（BD公司）测定IL-2、IL-4、IL-6、IL-10、TNFα和IFNγ的浓度。采用U-曼-惠特尼检验比较结果。
我们发现，研究组中IFNγ浓度在统计学上显著升高：8.4±5.3 pg/ml对比4.2±3.2 pg/ml（p = 0.02），TNFα：1.5±0.7 pg/ml对比0.7±0.3 pg/ml（p = 0.04），IL-2：1.3±0.6 pg/ml对比0.6±0.4 pg/ml（p = 0.01）。IL-6水平也有所升高，为35.5±21.0 pg/ml对比19.8±12.3 pg/ml，但差异未达到统计学显著性。两组中IL-4和IL-10的浓度相似。
先兆子痫妇女血清中Th1细胞因子（IFNγ、IL-2）浓度升高表明，在这种病理状态下血液的细胞毒性活性增强，同时伴有炎症细胞因子TNFα和IL-6水平升高。
