Dipartimento di Scienze e Tecnologie Veterinarie, per la Sicurezza Alimentare, Università di Milano, Milan, Italy.
Tissue Eng Part C Methods. 2010 Dec;16(6):1515-21. doi: 10.1089/ten.TEC.2010.0214. Epub 2010 Sep 6.
The adipose-derived stromal vascular fraction (SVF) represents a rich source of mesenchymal cells, potentially able to differentiate into adipocytes, chondrocytes, osteoblasts, myocytes, cardiomyocytes, hepatocytes, and neuronal, epithelial, and endothelial cells. These cells are ideal candidates for use in regenerative medicine, tissue engineering, including gene therapy, and cell replacement cancer therapies. In this work, we aimed to the optimization of the adipose SVF-based therapy, and the effect of the collection site, surgical procedure, and tissue processing techniques on SVF yield was evaluated in terms of cell recovery and live cells, taking into account the effect of gender, age, and body mass index. Adipose tissue samples were recovered from 125 informed subjects (37 males and 88 females; mean age: 51.31 years; range: 15-87 years), and digested in different condition with collagenase. A multivariate linear model put in evidence that in males the best collection site in terms of yield is located in the abdomen, whereas in females the biopsy region do not influence cell recovery; the collection technique, the age, and the body mass index of donor seem not to influence the cell yield. The tissue-processing procedures strongly modify the yield and the vitality of cells: a collagenase concentration of 0.2% and a digestion time of 1 h could be chosen as the best operating conditions.
脂肪组织来源的基质血管部分（SVF）代表了丰富的间充质细胞来源，具有潜在的分化为脂肪细胞、软骨细胞、成骨细胞、肌细胞、心肌细胞、肝细胞以及神经元、上皮细胞和内皮细胞的能力。这些细胞是再生医学、组织工程学（包括基因治疗和细胞替代癌症疗法）的理想候选物。在这项工作中，我们旨在优化基于脂肪 SVF 的治疗方法，并评估采集部位、手术程序和组织处理技术对 SVF 产量的影响，包括细胞回收和活细胞，同时考虑性别、年龄和体重指数的影响。从 125 名知情同意的受试者（37 名男性和 88 名女性；平均年龄：51.31 岁；范围：15-87 岁）中回收脂肪组织样本，并在不同条件下用胶原酶进行消化。多元线性模型表明，就产量而言，男性最佳的采集部位位于腹部，而女性的活检区域不影响细胞回收；供体的采集技术、年龄和体重指数似乎不影响细胞产量。组织处理程序强烈影响细胞的产量和活力：可以选择 0.2%的胶原酶浓度和 1 小时的消化时间作为最佳操作条件。
