Fraser J K, Wulur I, Alfonso Z, Zhu M, Wheeler E S
Cytori Therapeutics Inc, San Diego, CA 92121, USA.
Cytotherapy. 2007;9(5):459-67. doi: 10.1080/14653240701358460.
Human adipose tissue has been shown to contain multipotent cells with properties similar to mesenchymal stromal cells. While there have been many studies of the biology of these cells, no study has yet evaluated issues associated with tissue harvest.
Adipose tissue was obtained from the subcutaneous space of the abdomen and hips of 10 donors using both syringe and pump-assisted liposuction. Tissue was digested with collagenase and then assayed for the presence of different stem and progenitor cell types using clonogenic culture assays, including fibroblast colony-forming unit (CFU-F) and alkaline phosphatase-positive colony-forming unit (CFU-AP). Paired analysis of samples obtained from the same individual was used to compare harvest method and site.
Syringe suction provided significantly greater recovery of adipocytes and a non-significant trend towards improved recovery of cells in the adipocyte-depleted fraction. There was considerable donor-to-donor variation in stem cell recovery. However, paired analysis of tissue obtained from different subcutaneous sites in the same donor showed that tissue harvested from the hip yielded 2.3-fold more CFU-F/unit volume and a 7-fold higher frequency of CFU-AP than that obtained from the abdomen. These differences were statistically significant.
Harvest site influences the stem and progenitor cell content of subcutaneous adipose tissue.
已证明人类脂肪组织含有具有与间充质基质细胞相似特性的多能细胞。虽然对这些细胞的生物学特性已有许多研究，但尚未有研究评估与组织采集相关的问题。
使用注射器和泵辅助抽脂术从10名供体的腹部和臀部皮下间隙获取脂肪组织。用胶原酶消化组织，然后使用克隆形成培养试验检测不同干细胞和祖细胞类型的存在情况，包括成纤维细胞集落形成单位（CFU-F）和碱性磷酸酶阳性集落形成单位（CFU-AP）。对从同一个体获取的样本进行配对分析，以比较采集方法和部位。
注射器抽吸法获得的脂肪细胞回收率显著更高，且在脂肪细胞耗尽部分的细胞回收率有改善的非显著趋势。干细胞回收率在供体之间存在相当大的差异。然而，对同一供体不同皮下部位获取的组织进行配对分析表明，从臀部采集的组织每单位体积产生的CFU-F比从腹部采集的多2.3倍，CFU-AP频率高7倍。这些差异具有统计学意义。
采集部位会影响皮下脂肪组织的干细胞和祖细胞含量。
