Kawasaki N, Tanimoto T, Tanaka A
Division of Biological Chemistry and Biologicals, National Institute of Hygienic Sciences, Tokyo, Japan.
Anal Biochem. 1991 Jan;192(1):104-8. doi: 10.1016/0003-2697(91)90192-v.
A new, rapid, sensitive, and specific method combining ion chromatography with electrochemical detection was developed for measuring non-protein-bound Fe(II) and Fe(III) in biological samples. The procedure was based on the separation of the iron-diethylenetriaminepentaacetic acid complex formed directly on the chromatographic column with anion-exchange resin followed by electrochemical detection. The method enabled more than 0.5 microM Fe(II) and Fe(III) to be determined for injection volumes of 10 microliters. This method was applicable for the determination of Fe(II) and Fe(III) in ultrafiltrates of the rat liver cytosolic fraction. It was found that release of iron from iron-bound proteins was pH dependent and that non-protein-bound iron in the tissues was determined in a ferrous state at low pH values.
开发了一种将离子色谱与电化学检测相结合的新型、快速、灵敏且特异的方法，用于测定生物样品中的非蛋白结合铁（II）和铁（III）。该方法基于在阴离子交换树脂的色谱柱上直接形成的铁-二乙三胺五乙酸络合物的分离，随后进行电化学检测。对于10微升的进样体积，该方法能够测定超过0.5微摩尔的铁（II）和铁（III）。此方法适用于大鼠肝细胞溶质部分超滤物中铁（II）和铁（III）的测定。发现铁从铁结合蛋白中的释放依赖于pH值，并且在低pH值下组织中的非蛋白结合铁以亚铁状态测定。
