Conyers S M, Kidwell D A
Chemistry Division, Naval Research Laboratory, Washington, D.C. 20375-5000.
Anal Biochem. 1991 Jan;192(1):207-11. doi: 10.1016/0003-2697(91)90208-b.
Two new detection systems for horseradish peroxidase (HRP) have been developed for the staining of membranes used in immunoassays. These systems use dimethyl or diethyl analogues of p-phenylenediamine with 4-chloro-1-naphthol to generate a blue product or 3-methyl-2-benzothiazolinone hydrazone with 4-chloro-1-naphthol to generate a red product. These reagents offer increased sensitivity and lower background staining than currently available chromogenic detection substrates. In addition, the incorporation of these substrates increases the sensitivity of HRP labels to be comparable to that of alkaline phosphatase with the 5-bromo-4-chloro-3-indolyl phosphate + nitro blue tetrazolium substrate.
已开发出两种用于辣根过氧化物酶（HRP）的新型检测系统，用于免疫分析中膜的染色。这些系统使用对苯二胺的二甲基或二乙基类似物与4-氯-1-萘酚生成蓝色产物，或使用3-甲基-2-苯并噻唑啉酮腙与4-氯-1-萘酚生成红色产物。与目前可用的显色检测底物相比，这些试剂具有更高的灵敏度和更低的背景染色。此外，这些底物的加入使HRP标记的灵敏度提高，可与使用5-溴-4-氯-3-吲哚磷酸+硝基蓝四唑底物的碱性磷酸酶相媲美。
