肯尼亚内脏利什曼病诊断中利什曼原虫寡聚 C 测试和 NASBA-寡聚层析法的敏感性和特异性。
Sensitivity and specificity of the Leishmania OligoC-TesT and NASBA-oligochromatography for diagnosis of visceral leishmaniasis in Kenya.
机构信息
Centre for Clinical Research, Kenya Medical Research Institute, Nairobi, Kenya.
出版信息
Trop Med Int Health. 2010 Jul;15(7):806-10. doi: 10.1111/j.1365-3156.2010.02548.x. Epub 2010 May 14.
OBJECTIVE
To estimate the sensitivity and specificity of the OligoC-TesT and nucleic acid sequence-based amplification coupled to oligochromatography (NASBA-OC) for molecular detection of Leishmania in blood from patients with confirmed visceral leishmaniasis (VL) and healthy endemic controls from Kenya.
METHODS
Blood specimens of 84 patients with confirmed VL and 98 endemic healthy controls from Baringo district in Kenya were submitted to both assays.
RESULTS
The Leishmania OligoC-TesT showed a sensitivity of 96.4% (95% confidence interval [CI]: 90-98.8%) and a specificity of 88.8% (95% CI: 81-93.6%), while the sensitivity and specificity of the NASBA-OC were 79.8% (95% CI: 67-87%) and 100% (95% CI: 96.3-100%), respectively.
CONCLUSION
Our findings indicate high sensitivity of the Leishmania OligoC-TesT on blood while the NASBA-OC is a better marker for active disease.
目的
评估寡核苷酸连接酶检测试验（OligoC-TesT）和核酸序列扩增结合寡核苷酸层析技术（NASBA-OC）在检测肯尼亚经确认的内脏利什曼病（VL）患者和健康流行区对照者血液中的利什曼原虫的灵敏度和特异性。
方法
对来自肯尼亚巴林戈区的 84 例经确认 VL 患者和 98 例流行区健康对照者的血样标本进行了这两种检测。
结果
利什曼原虫寡核苷酸连接酶检测试验的灵敏度为 96.4%（95%可信区间：90-98.8%），特异性为 88.8%（95%可信区间：81-93.6%），而 NASBA-OC 的灵敏度和特异性分别为 79.8%（95%可信区间：67-87%）和 100%（95%可信区间：96.3-100%）。
结论
我们的研究结果表明，利什曼原虫寡核苷酸连接酶检测试验在血液检测方面具有很高的灵敏度，而 NASBA-OC 则是活动性疾病的更好标志物。
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