Li Yan, Wang Yi-qin, Li Ming, Zhang Ying, Li Fu-rong, Wang Zi-hua, Qi Wei, Yuan Fa-huan
Department of Nephrology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2010 Jun;26(6):522-4.
To investigate the relationship between costimulatory molecule VSIG4 expression in macrophage and mice renal interstitial fibrosis.
A total of 40 healthy male C57B6 mice (VSIG4(-/-); and VSIG4(+/+);) were divided into two groups, VSIG4(-/-); tubulointerstitial Nephritis (n=20) and VSIG4(+/+); tubulointerstitial Nephritis (n=20), mice received left urethral obstruction operation(UUO) then were killed at 7 d before operation, 3 d, 7 d and 14 d post operation for both groups.Blood SCr and BUN were detected. Renal interstitial fibrosis was measured by HE stain. The expression of TGF-beta1, MMP-2, in UUO mice(VSIG4(-/-); and VSIG4(+/+);) were detected by immunohistochemistry.
SCr and BUN levels in the two groups have no significantly different. TGF-beta1 was significantly increased in the VSIG4(-/-); UUO mice group in comparison with the other group, while MMP-2 was reduced.
The interstitial inflammatory cell infiltration and tubular lesion of VSIG4(-/-); UUO mice were increased, with the expression of TGF-beta1 increased and MMP-2 reduced. VSIG4 may play a role in inhibiting interstitial fibrosis.
探讨巨噬细胞共刺激分子VSIG4表达与小鼠肾间质纤维化的关系。
将40只健康雄性C57B6小鼠（VSIG4(-/-)；和VSIG4(+/+)；）分为两组，VSIG4(-/-)；肾小管间质性肾炎组（n=20）和VSIG4(+/+)；肾小管间质性肾炎组（n=20），两组小鼠均行左侧输尿管梗阻手术（UUO），并于术前7天、术后3天、7天和14天处死。检测血肌酐和尿素氮。通过HE染色测量肾间质纤维化程度。采用免疫组化法检测UUO小鼠（VSIG4(-/-)；和VSIG4(+/+)；）中TGF-β1、MMP-2的表达。
两组血肌酐和尿素氮水平无显著差异。与另一组相比，VSIG4(-/-)；UUO小鼠组TGF-β1显著升高，而MMP-2降低。
VSIG4(-/-)；UUO小鼠的间质炎性细胞浸润和肾小管病变增加，TGF-β1表达增加，MMP-2表达降低。VSIG4可能在抑制间质纤维化中发挥作用。
