Department of Radiology and Division of Molecular Therapy, Advanced Clinical Research Center, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo, Japan.
Mol Imaging. 2010 Jun;9(3):163-72.
We developed an integrated imaging method using bioluminescence imaging (BLI) and magnetic resonance imaging (MRI) and applied it to mouse tumor monitoring. BLIs and MRIs were successively acquired from mice fixed on a transparent flat plate and were registered using three small markers. After spatial coregistration, the two-dimensional BLI was fused with all MRI slices. The accuracy of image registration was evaluated using fluorescence imaging and MRI of many markers attached to the mice. Mice were inoculated with luciferase-expressing tumor cells subcutaneously or directly into the liver, and disease progression was evaluated repeatedly by integrated BLI-MRI. Image registration was achieved with acceptable accuracy, although the registration error increased with increasing distance from the center of the imaging field and was larger in imaging the convex dorsal side of the mouse than in imaging the flat ventral side. Integrated BLI-MRI allowed longitudinal assessment of disease progression and appeared to facilitate detailed interpretation of the imaging findings. It aided the anatomic localization of lesions demonstrated by BLI and recognition of MRI abnormalities corresponding to BLI foci. Integrated BLI-MRI is feasible and appears to be a practical method for comprehensive, longitudinal assessment of disease model mice.
我们开发了一种整合的成像方法，结合使用生物发光成像（BLI）和磁共振成像（MRI），并将其应用于小鼠肿瘤监测。BLIs 和 MRIs 从固定在透明平板上的小鼠中连续获取，并使用三个小标记进行注册。在空间配准后，二维 BLI 与所有 MRI 切片融合。通过将许多附着在小鼠上的荧光标记物的荧光成像和 MRI 来评估图像配准的准确性。将表达荧光素酶的肿瘤细胞皮下或直接接种到肝脏中，通过整合的 BLI-MRI 反复评估疾病进展。尽管注册误差随着成像场中心的距离增加而增加，并且在对小鼠凸面背侧成像时比在对平坦腹侧成像时更大，但图像配准达到了可接受的准确性。整合的 BLI-MRI 允许对疾病进展进行纵向评估，并且似乎有助于对成像结果进行详细解释。它有助于对 BLI 显示的病变进行解剖定位，并识别与 BLI 焦点相对应的 MRI 异常。整合的 BLI-MRI 是可行的，并且似乎是一种用于全面、纵向评估疾病模型小鼠的实用方法。
