乙酰胆碱酯酶——酰基酶中间体。

Acetylcholinesterase - the acyl-enzyme intermediate.

作者信息

Froede H C, Wilson I B

机构信息

Univ. of Colorado, Dept. of Chemistry Boulder, Colorado 80309 U.S.A.

出版信息

Neurochem Int. 1980;2C:193-7. doi: 10.1016/0197-0186(80)90024-8.

DOI:10.1016/0197-0186(80)90024-8

PMID:20487783

Abstract

The origin of the acetyl-enzyme theory for hydrolytic reactions of acetylcholinesterase is outlined. The relationship of this theory to the inhibition of acetylcholinesterase by organophosphates is emphasized. The steady state concentration of the acetyl-enzyme was measured using acetate labeled, tritrated acetylcholine as substrate. From pH 6 to pH 9.0, 60% to 70% of the 18S enzyme is labeled at 22 degrees C in high ionic strength.

摘要

概述了乙酰胆碱酯酶水解反应的乙酰酶理论的起源。强调了该理论与有机磷酸酯对乙酰胆碱酯酶抑制作用的关系。使用用氚标记的醋酸盐乙酰胆碱作为底物来测量乙酰酶的稳态浓度。在22摄氏度、高离子强度条件下，从pH 6到pH 9.0，18S酶的60%至70%被标记。