Aguilar J S, Lunt G G
Department of Biochemistry, University of Bath, Bath BA2 7AY, U.K.
Neurochem Int. 1984;6(4):501-7. doi: 10.1016/0197-0186(84)90121-9.
A crude membrane preparation from the supraoesophageal ganglion of the locust (Schistocerca gregaria) shows specific binding of muscarinic cholinergic ligands. Analysis of the kinetics of binding reveals the presence of at least two binding sites with dissociation constants, K(d) of 0.76 and 37.7 nM. The pharmacological profile of the higher affinity site is different from that seen for muscarinic receptor sites in mammalian brain. The binding sites reported here are quite distinct from nicotinic-like receptor sites in the same tissue and lend further support to suggestions that there are at least two types of acetylcholine receptors in insects.
从蝗虫（沙漠蝗）的食管上神经节制备的粗制膜制剂显示出对毒蕈碱型胆碱能配体的特异性结合。结合动力学分析表明存在至少两个结合位点，其解离常数K(d)分别为0.76和37.7 nM。高亲和力位点的药理学特征与哺乳动物脑中的毒蕈碱受体位点不同。本文报道的结合位点与同一组织中的烟碱样受体位点截然不同，并进一步支持了昆虫中至少存在两种乙酰胆碱受体的观点。
