Inmunología y Genética Aplicada S.A. (INGENASA), Hnos. García Noblejas, Madrid, Spain.
J Virol Methods. 2010 Sep;168(1-2):170-6. doi: 10.1016/j.jviromet.2010.05.011. Epub 2010 May 19.
Human respiratory syncytial virus (hRSV) is the main viral cause of severe respiratory infections in children and a common cause of morbidity in the elderly. The nucleocapsid (N) and fusion (F) proteins of hRSV were expressed in insect cells and used as antigens in two independent enzyme-linked immunosorbent assays (ELISAs) to measure the serum antibody response in two populations at high risk of hRSV infection, children and the elderly. Fifty-seven serum specimens from children aged from 1 to 10 years old and 91 sera from adults over 60 years old were tested. The ELISA results were compared with those obtained by an immunofluorescence assay (IFA) based on hRSV-infected cells, which was considered as the reference technique. Sensitivity and specificity were 94% and 85% for the N-ELISA and 86% and 81% for the F-ELISA, respectively. When the immune responses of the two groups of individuals were compared, it appeared that almost 100% of the elderly had antibodies against the N or F protein whereas only 50% of the sera from children had antibodies against either of the two viral proteins. In conclusion, the F and N ELISAs can be used successfully for detecting a specific antibody response to hRSV.
人呼吸道合胞病毒（hRSV）是导致儿童严重呼吸道感染的主要病毒病原体，也是老年人发病率高的常见原因。我们在昆虫细胞中表达了 hRSV 的核衣壳（N）和融合（F）蛋白，并将其分别作为两种独立酶联免疫吸附试验（ELISA）的抗原，以检测两个高风险 RSV 感染人群，即儿童和老年人的血清抗体反应。我们检测了 57 份 1 至 10 岁儿童的血清样本和 91 份 60 岁以上成年人的血清样本。ELISA 结果与基于 hRSV 感染细胞的免疫荧光分析（IFA）获得的结果进行了比较，后者被认为是参考技术。N-ELISA 的敏感性和特异性分别为 94%和 85%，F-ELISA 的敏感性和特异性分别为 86%和 81%。当比较两组个体的免疫反应时，几乎 100%的老年人对 N 或 F 蛋白有抗体，而只有 50%的儿童血清对两种病毒蛋白中的任何一种有抗体。总之，F 和 N ELISA 可成功用于检测针对 hRSV 的特异性抗体反应。
