Maifeld Sarah V, Ro Bodrey, Mok Hoyin, Chu Marla, Yu Li, Yamagata Ryan, Leonardson Tansy, Chio Vera, Parhy Bandita, Park Samuel, Carlson Marcia, Machhi Shushil, Ulbrandt Nancy, Falsey Ann R, Walsh Edward E, Wang C Kathy, Esser Mark T, Zuo Fengrong
Applied Immunology and Microbiology, MedImmune, Mountain View, California, United States of America.
Non-clinical Biostatistics, MedImmune, Gaithersburg, Maryland, United States of America.
PLoS One. 2016 Apr 12;11(4):e0153019. doi: 10.1371/journal.pone.0153019. eCollection 2016.
Sensitive and precise serology assays are needed to measure the humoral response to antigens of respiratory syncytial virus (RSV) following natural infection or vaccination. We developed and evaluated a collection of electrochemiluminescent (ECL) serology assays using four RSV antigens (F, N, Ga and Gb). To assess the merits of ECL technology, the four ECL serology assays were evaluated using a well-characterized "gold standard" panel of acute and convalescent serum samples from fifty-nine RSV-positive and thirty RSV-negative elderly subjects (≥65 years old). The combined results from the four ECL assays demonstrated good concordance to the "gold standard" diagnosis, reaching 95% diagnostic sensitivity and 100% diagnostic specificity. Additionally, a combination of ECL assays provided higher diagnostic sensitivity than a commercially available diagnostic ELISA or cell-based microneutralization assay. In summary, these data demonstrate the advantages of using ECL-based serology assays and highlight their use as a sensitive diagnostic approach to detect recent RSV infection in an elderly population.
需要灵敏且精确的血清学检测方法来测量自然感染或接种疫苗后对呼吸道合胞病毒（RSV）抗原的体液免疫反应。我们开发并评估了一系列使用四种RSV抗原（F、N、Ga和Gb）的电化学发光（ECL）血清学检测方法。为了评估ECL技术的优点，使用来自59名RSV阳性和30名RSV阴性老年受试者（≥65岁）的一组经过充分表征的急性和恢复期血清样本作为“金标准”，对这四种ECL血清学检测方法进行了评估。四种ECL检测方法的综合结果与“金标准”诊断显示出良好的一致性，诊断敏感性达到95%，诊断特异性达到100%。此外，ECL检测方法的组合比市售诊断ELISA或基于细胞的微量中和试验具有更高的诊断敏感性。总之，这些数据证明了使用基于ECL的血清学检测方法的优势，并突出了它们作为一种灵敏的诊断方法用于检测老年人群近期RSV感染的用途。
