Department of Chemistry, King Fahd University of Petroleum and Minerals, Dhahran 31261, Saudi Arabia.
J Chromatogr A. 2010 Oct 22;1217(43):6661-7. doi: 10.1016/j.chroma.2010.04.066. Epub 2010 May 18.
The simultaneous extraction of acidic and basic analytes from a particular sample is a challenging task. In this work, electromembrane extraction (EME) of acidic non-steroidal anti-inflammatory drugs and basic β-blockers in a single step was carried out for the first time. It was shown that by designing an appropriate compartmentalized membrane envelope, the two classes of drugs could be electrokinetically extracted by a 300 V direct current electrical potential. This method required only a very short 10-min extraction time from a pH-neutral sample, with a small amount (50 μL) of organic solvent (1-octanol) as the acceptor phase. Analysis was carried out using gas chromatography-mass spectrometry after derivatization of the analytes. Extraction parameters such as extraction time, applied voltage, pH range, and concentration of salt added were optimized. The proposed EME technique provided good linearity with correlation coefficients from 0.982 to 0.997 over a concentration range of 1-200 μg L⁻¹. Detection limits of the drugs ranged between 0.0081 and 0.26 μg L⁻¹, while reproducibility ranged from 6 to 13% (n=6). Finally, the application of the new method to wastewater samples was demonstrated.
同时从特定样品中提取酸性和碱性分析物是一项具有挑战性的任务。在这项工作中，首次在一步法中进行了电膜萃取（EME）酸性非甾体抗炎药和碱性β受体阻滞剂。结果表明，通过设计适当的分隔膜包封，这两类药物可以通过 300 V 直流电场进行电动提取。该方法仅需从 pH 中性样品中进行非常短的 10 分钟提取，使用少量（50 μL）有机溶剂（1-辛醇）作为接受相。在对分析物进行衍生化后，使用气相色谱-质谱法进行分析。优化了萃取时间、施加电压、pH 值范围和添加盐的浓度等萃取参数。所提出的 EME 技术提供了良好的线性度，相关系数在 1-200 μg L⁻¹ 浓度范围内从 0.982 到 0.997。药物的检测限在 0.0081 到 0.26 μg L⁻¹ 之间，而重现性在 6%到 13%（n=6）之间。最后，证明了该新方法在废水样品中的应用。
