Department of Microbiology, BMS Block, Panjab University, Chandigarh 160014, India.
J Med Microbiol. 2010 Aug;59(Pt 8):913-919. doi: 10.1099/jmm.0.020107-0. Epub 2010 May 20.
Intranasal (i.n.) instillation of different amounts of purified Stenotrophomonas maltophilia flagellin preparation (1, 5 and 15 microg) in BALB/c mice stimulated a transient innate immune response in the lungs. This was characterized by infiltration of different kinds of leukocytes (neutrophils, monocytes and lymphocytes), production of various inflammatory mediators (tumour necrosis factor alpha, interleukin 1 beta, interleukin 10, nitric oxide, myeloperoxidase and malondialdehyde) and activated alveolar macrophages (AMs). The proinflammatory cytokine production resulted in accumulation of activated neutrophils and macrophages and their products following immunostimulation with flagellin. The activation of AMs by flagellin was non-specific as AMs obtained from flagellin-treated animals, even after 4 h of exposure, were found to engulf and kill S. maltophilia and Staphylococcus aureus efficiently compared to macrophages obtained from control animals. i.n. instillation of 5 microg flagellin resulted in the generation of an effective innate immunity compared to other flagellin doses. Our data provide strong evidence that S. maltophilia flagellin stimulates innate immunity in mouse lung.
鼻腔（i.n.）内滴注不同剂量的纯化嗜麦芽寡养单胞菌鞭毛蛋白制剂（1、5 和 15 微克）可刺激 BALB/c 小鼠肺部的短暂先天免疫反应。这表现为不同类型的白细胞（中性粒细胞、单核细胞和淋巴细胞）浸润、产生各种炎症介质（肿瘤坏死因子-α、白细胞介素 1β、白细胞介素 10、一氧化氮、髓过氧化物酶和丙二醛）以及肺泡巨噬细胞（AMs）激活。在鞭毛蛋白免疫刺激后，促炎细胞因子的产生导致活化的中性粒细胞和巨噬细胞及其产物的积累。鞭毛蛋白对 AMs 的激活是非特异性的，因为即使在暴露 4 小时后，从鞭毛蛋白处理的动物中获得的 AMs 也被发现能够有效地吞噬和杀死嗜麦芽寡养单胞菌和金黄色葡萄球菌，与从对照动物中获得的巨噬细胞相比。与其他鞭毛蛋白剂量相比，5 微克鞭毛蛋白的鼻腔内滴注可产生有效的先天免疫。我们的数据提供了强有力的证据表明嗜麦芽寡养单胞菌鞭毛蛋白可刺激小鼠肺部的先天免疫。
