Xu Huacheng, He Pinjing, Wang Guanzhao, Shao Liming
State Key Laboratory of Pollution Control and Resource Reuse, Key Laboratory of Yangtze River Water Environment, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
Water Sci Technol. 2010;61(11):2931-42. doi: 10.2166/wst.2010.197.
Three-dimensional excitation emission matrix (EEM) fluorescence spectroscopy and gel-permeating chromatography (GPC) were employed to characterize the extracellular polymeric substances (EPS) in aerobic granulation. EPS matrix in this study was stratified into four fractions: (1) supernatant, (2) slime, (3) loosely bound EPS (LB-EPS), and (4) tightly bound EPS (TB-EPS). The results showed that the dissolved organic carbon was mainly distributed in TB-EPS fraction, and increased with increasing the operating time. The supernatant, slime, and LB-EPS fractions exhibited four fluorescence peaks, an autochthonous signature, unimodal MW distribution and lower molecular weight (MW) (3 < log [MW]<5), whereas the TB-EPS fraction only had two peaks, an allochthonous signature, multiple peaks and higher MW (5 < log [MW]<7). It was deemed that the formation of aerobic granules was correlated with the accumulation of proteins in the TB-EPS fraction. EEM spectroscopy and GPC profiles could be used as appropriate and effective methods to characterize the EPS in aerobic granulation from a micro-view level.
采用三维激发发射矩阵（EEM）荧光光谱法和凝胶渗透色谱法（GPC）对好氧颗粒污泥中的胞外聚合物（EPS）进行表征。本研究中的EPS基质分为四个部分：（1）上清液，（2）黏液，（3）松散结合的EPS（LB-EPS），以及（4）紧密结合的EPS（TB-EPS）。结果表明，溶解有机碳主要分布在TB-EPS部分，且随运行时间的增加而增加。上清液、黏液和LB-EPS部分呈现四个荧光峰，具有自生源特征、单峰分子量分布且分子量较低（3 < log [MW]<5），而TB-EPS部分仅有两个峰，具有异生源特征、多峰且分子量较高（5 < log [MW]<7）。认为好氧颗粒的形成与TB-EPS部分中蛋白质的积累有关。EEM光谱和GPC图谱可作为从微观层面表征好氧颗粒污泥中EPS的合适且有效的方法。
