Knapp B, Hundt E, Enders B, Küpper H A
Research Laboratories of Behringwerke AG, Marburg, Germany.
Behring Inst Mitt. 1991 Feb(88):147-56.
Based on investigations on several blood stage antigens from Plasmodium falciparum we have expressed a hybrid protein in E. coli containing 262 amino acids of the serine-stretch protein SERP and 189 amino acids of the histidine alanine rich protein HRPII. Antibodies raised against the hybrid protein by immunization of rabbits and Aotus monkeys react with both corresponding schizont polypeptides. Two Aotus monkeys immunized with the SERP/HRPII hybrid protein showed only low parasitemias after challenge infection with P. falciparum, compared to the control group. The result suggests that hybrid proteins of this type may be the basis for the development of a malaria vaccine.
基于对恶性疟原虫几种血液阶段抗原的研究，我们在大肠杆菌中表达了一种杂交蛋白，该蛋白包含丝氨酸延伸蛋白SERP的262个氨基酸和富含组氨酸丙氨酸的蛋白HRPII的189个氨基酸。通过免疫兔子和夜猴产生的针对该杂交蛋白的抗体与相应的裂殖体多肽都发生反应。与对照组相比，用SERP/HRPII杂交蛋白免疫的两只夜猴在受到恶性疟原虫攻击感染后仅表现出低水平的寄生虫血症。结果表明，这种类型的杂交蛋白可能是开发疟疾疫苗的基础。
