Enders B, Hundt E, Knapp B
Research Laboratories of Behringwerke AG, Marburg, Germany.
Vaccine. 1992;10(13):920-7. doi: 10.1016/0264-410x(92)90326-f.
Susceptible Aotus monkeys were immunized with Escherichia coli-derived fusion proteins containing partial sequences of the proteins MSAI, SERP, HRPII and with a group of three recombinant antigens isolated by screening with an antiserum raised against the protective 41 kDa protein band. HRPII, the combination of the fusion proteins of the 41 kDa group and a mixture of two sequences of SERP conferred significant protection against a challenge infection with Plasmodium falciparum blood stages. Based on the protective capacity of these recombinant antigens we have expressed two hybrid proteins (MS2/SERP/HRPII and SERP/MSAI/HRPII) in E. coli containing selected partial sequences. In two independent immunization trials it was shown that immunization of Aotus monkeys with either of the two hybrid proteins can protect the animals from an experimental P. falciparum infection.
将易感染的夜猴用含有疟原虫裂殖子表面蛋白1（MSAI）、富含半胱氨酸和组氨酸的蛋白质（SERP）、疟原虫富含组氨酸蛋白II（HRPII）部分序列的大肠杆菌衍生融合蛋白，以及通过用针对保护性41 kDa蛋白条带产生的抗血清筛选分离出的一组三种重组抗原进行免疫。HRPII、41 kDa组融合蛋白的组合以及SERP的两个序列的混合物对恶性疟原虫血液阶段的攻击感染提供了显著的保护。基于这些重组抗原的保护能力，我们在大肠杆菌中表达了两种含有选定部分序列的杂合蛋白（MS2/SERP/HRPII和SERP/MSAI/HRPII）。在两项独立的免疫试验中表明，用这两种杂合蛋白中的任何一种对夜猴进行免疫都可以保护动物免受实验性恶性疟原虫感染。
