College of Environmental Sciences and Engineering, Peking University, Beijing 100871, People's Republic of China.
Appl Microbiol Biotechnol. 2010 Aug;87(5):1943-51. doi: 10.1007/s00253-010-2670-8. Epub 2010 May 21.
Two pyridine-degrading bacteria and two quinoline-degrading bacteria were introduced for bioaugmentation to treat the coking wastewater. Sequencing batch reactors (SBRs) were used for a comparative study on the treatment efficiency of pyridine, quinoline, and chemical oxygen demand. Results showed that the treatment efficiency with coking-activated sludge plus a mixture of the four degrading bacteria was much better than that ones with coking-activated sludge only or mixed degrading bacteria only. Moreover, a 52-day continuous operation of the bioaugmented and general SBRs was investigated. The bioaugmented SBR showed better treatment efficiency and stronger capacity to treat high pyridine and quinoline shock loading. The general SBR failed to cope with the shock loading, and the biomass of the activated sludge decreased significantly. In order to monitor the microbial ecological variation during the long-term treatment, the bacterial community in both reactors was monitored by the amplicon length heterogeneity polymerase chain reaction technique. The diversity of the bacterial community decreased in both reactors, but the introduced highly efficient bacteria were dominant in the bioaugmented SBR. Our experiment showed clearly that the use of highly efficient bacteria in SBR process could be a feasible method to treat wastewater containing pyridine or/and quinoline.
引入了两种吡啶降解菌和两种喹啉降解菌进行生物强化，以处理焦化废水。采用序批式反应器（SBR）比较了吡啶、喹啉和化学需氧量的处理效率。结果表明，在焦化活性污泥中添加四种降解菌的混合物的处理效率明显优于仅添加焦化活性污泥或混合降解菌的处理效率。此外，对生物强化和常规 SBR 进行了 52 天的连续运行。生物强化 SBR 表现出更好的处理效率和更强的处理高浓度吡啶和喹啉冲击负荷的能力。常规 SBR 无法应对冲击负荷，活性污泥的生物量明显减少。为了监测长期处理过程中的微生物生态变化，采用扩增子长度异质性聚合酶链反应技术监测了两个反应器中的细菌群落。两个反应器中的细菌群落多样性都有所下降，但在生物强化 SBR 中，引入的高效细菌占据优势。我们的实验清楚地表明，在 SBR 工艺中使用高效细菌是处理含吡啶或/和喹啉废水的一种可行方法。
