Yagi K, Koshizaka T, Kito M, Ozawa T, Nishikimi M
Institute of Applied Biochemistry, Yagi Memorial Park, Japan.
Biochem Biophys Res Commun. 1991 Jun 14;177(2):659-63. doi: 10.1016/0006-291x(91)91839-5.
L-Gulono-gamma-lactone oxidase (GLO), an enzyme that is missing in scurvy-prone animals, was produced in COS-1 cells by transfection with a minigene constructed from pSVL vector and rat GLO cDNA. A functional GLO protein was expressed, which was indistinguishable from rat GLO in molecular size. The microsomes obtained from the transfected COS-1 cells showed GLO activity comparable to that in rat liver microsomes.
L-古洛糖酸-γ-内酯氧化酶（GLO）是一种在易患坏血病的动物体内缺失的酶，通过用由pSVL载体和大鼠GLO cDNA构建的小基因转染COS-1细胞而在其中产生。表达了一种功能性GLO蛋白，其分子大小与大鼠GLO无法区分。从转染的COS-1细胞中获得的微粒体显示出与大鼠肝脏微粒体相当的GLO活性。
