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Gene mapping and gene enrichment by the avidin-biotin interaction: use of cytochrome-c as a polyamine bridge.

作者信息

Sodja A, Davidson N

出版信息

Nucleic Acids Res. 1978 Feb;5(2):385-401. doi: 10.1093/nar/5.2.385.

DOI:10.1093/nar/5.2.385
PMID:204910
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC341990/
Abstract

A modification of previously described methods of electron microscopic gene mapping and of gene enrichment based on the avidin-biotin interaction is presented. The modification consists of coupling cytochrome-c instead of pentane diamine to the oxidized 2', 3' terminus of an RNA by Schiff base formation and BH-4 reduction. The RNA-cytochrome-c conjugate is purified by a simple chromatographic procedure; several biotins are attached to the cytochrome moiety by acylation. The extended arm between biotin and RNA gives efficient electron microscopic gene mapping of DNA:RNA-biotin hybrids with avidin-ferritin and avidin-polymethacylate sphere labels and efficient gene enrichment by buoyant banding of DNA:RNA-biotin:avidin-spheres in CsCl. A 1400 fold enrichment (thus, 25% pure) and a 90% yield of long Drosophila DNA strands with 5S RNA genes is achieved.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce99/341990/7d940ad9d4e2/nar00463-0097-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce99/341990/7abec06702bc/nar00463-0094-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce99/341990/7d940ad9d4e2/nar00463-0097-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce99/341990/7abec06702bc/nar00463-0094-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce99/341990/7d940ad9d4e2/nar00463-0097-a.jpg

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Synthesis of 8-(2-4 dinitrophenyl 2-6 aminohexyl) amino-adenosine 5' triphosphate: biological properties and potential uses.8 -（2 - 4 -二硝基苯基 - 2 - 6 -氨基己基）氨基 - 腺苷5'-三磷酸的合成：生物学特性及潜在用途

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Use of nonisotopic M13 probes for genetic analysis: application to HLA class II loci.非同位素M13探针在基因分析中的应用：应用于HLA II类基因座
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An electron microscope study of the relative positions of the 4S and ribosomal RNA genes in HeLa cells mitochondrial DNA.对海拉细胞线粒体DNA中4S和核糖体RNA基因相对位置的电子显微镜研究。
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Restriction map of 5S RNA genes of Drosophila melanogaster.黑腹果蝇5S RNA基因的限制酶切图谱。
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