Hematology Laboratory, Hospital Galdakao-Usansolo, Galdakao, Vizcaya, Spain.
Int J Lab Hematol. 2011 Feb;33(1):37-44. doi: 10.1111/j.1751-553X.2010.01238.x.
The reticulocyte hemoglobin equivalent (Ret He) represents an indirect measure of the functional iron available for erythropoiesis over the previous 2-3 days. Only the analyzers of a single manufacturer, Sysmex (Sysmex Corporation, Kobe, Japan), include Ret He. Red blood cell size factor (RSf) is a new parameter provided by Beckman Coulter, which joins together the volume of the erythrocytes and the volume of reticulocytes. The aims of the study were to investigate the clinical usefulness of RSf in the study of erythropoiesis status and to assess its concordance with Ret He values. Samples from 417 patients were run on both LH 780 (Beckman Coulter) and Sysmex XE 5000 analyzers. Independent samples t-test, Pearson correlation, receiver operating characteristic (ROC) analysis and inter-rater reliability (κ index) were applied. Good correlation between RSf and Ret He was observed, r = 0.8184. Significant differences (P < 0.001) were detected when groups with inefficient erythropoiesis were compared with patients undergoing therapy and healthy subjects. ROC analysis for RSf in the diagnosis of inefficient erythropoiesis, cutoff 91.1 fl, area under curve 0.963, sensitivity 91.7%, specificity 88.5%. Concordance between RSf and Ret He κ = 0.68. RSf and Ret He are suitable parameters for the assessment of erythropoiesis status.
网织红细胞血红蛋白含量（Ret-He）代表了过去 2-3 天内用于红细胞生成的功能性铁的间接测量。只有单一制造商 Sysmex（日本神户市的 Sysmex 公司）的分析仪包括 Ret-He。红细胞大小因子（RSf）是贝克曼库尔特提供的一个新参数，它将红细胞体积和网织红细胞体积结合在一起。本研究的目的是探讨 RSf 在研究红细胞生成状态中的临床应用，并评估其与 Ret-He 值的一致性。将 417 例患者的样本分别在 LH 780（贝克曼库尔特）和 Sysmex XE 5000 分析仪上进行检测。应用独立样本 t 检验、Pearson 相关、受试者工作特征（ROC）分析和组内一致性（κ 指数）。RSf 与 Ret-He 之间存在良好的相关性，r = 0.8184。当比较低效红细胞生成组与接受治疗的患者和健康受试者时，检测到显著差异（P < 0.001）。RSf 用于诊断低效红细胞生成的 ROC 分析，截断值 91.1 fl，曲线下面积 0.963，灵敏度 91.7%，特异性 88.5%。RSf 与 Ret-He 的一致性 κ = 0.68。RSf 和 Ret-He 是评估红细胞生成状态的合适参数。
