US Dept. of Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 E. Mermaid Lane, Wyndmoor, PA 19038, USA.
J Food Sci. 2010 Mar;75(2):E110-5. doi: 10.1111/j.1750-3841.2009.01482.x.
A new microwave heating process was developed for cooking microwaveable foods containing raw meats. A commercially available inverter-based microwave oven was modified for pasteurization of mechanically tenderized beef, inoculated with Escherichia coli O157:H7 (approximately 5 log(10) CFU/g) and packaged in a 12-oz CPET tray containing 150-mL de-ionized water. The new microwave heating system was equipped with an infrared sensor and a proportional feedback mechanism to allow temperature controlled microwave heating. A 2-stage heating strategy was adopted to cook the product. In the primary heating stage, the sample surface temperature was increased to an initial temperature set-point (ITSP, 65, 70, 75, or 80 degrees C). In the secondary heating stage, the heating was continued with a small fraction of microwave power. The effect of ITSP, hold time (0 to 3 min), and sample elevation (0, 0.03, and 0.07 m above turntable) on inactivation of E. coli O157:H7 and background microflora was evaluated. It was observed that only a small number (approximately 1.3 logs) of E. coli O157:H7 and background microflora were inactivated in the primary heating stage. The elevation 0.07 m, which was in the proximity of the geometric center of the metal cavity, was more effective in inactivating both E. coli O157:H7 and background microflora. Substantially more bacteria were inactivated in the secondary heating stage. Complete inactivation of E. coli and background microflora was observed with heating at temperatures above 70 degrees C for more than 1 min. This study demonstrated a new approach for ensuring the safety of microwaveable products containing raw meats.
一种新的微波加热工艺被开发用于烹饪含有生肉的微波食品。一台市售的基于逆变器的微波炉经过改装,用于巴氏消毒机械嫩化的牛肉,接种大肠杆菌 O157:H7(约 5log(10)CFU/g),并包装在一个含有 150 毫升去离子水的 12 盎司 CPET 托盘内。新的微波加热系统配备了一个红外传感器和一个比例反馈机制,以实现温度控制的微波加热。采用两阶段加热策略来烹饪产品。在初级加热阶段,将样品表面温度升高到初始温度设定点(ITSP,65、70、75 或 80°C)。在二级加热阶段,用一小部分微波功率继续加热。研究了 ITSP、保持时间(0 至 3 分钟)和样品高度(旋转台上方 0、0.03 和 0.07 米)对大肠杆菌 O157:H7 和背景微生物的灭活效果。结果表明,在初级加热阶段,只有少数(约 1.3 个对数)大肠杆菌 O157:H7 和背景微生物被灭活。高度为 0.07 米,接近金属腔的几何中心,对大肠杆菌 O157:H7 和背景微生物的灭活效果更显著。在二级加热阶段,更多的细菌被灭活。在温度高于 70°C 加热超过 1 分钟时,观察到大肠杆菌和背景微生物的完全灭活。本研究为确保含有生肉的微波食品的安全性提供了一种新方法。