The simultaneous quantification of dopamine, norepinephrine and epinephrine in micropunched rat brain nuclei by on-line trace enrichment HPLC with electrochemical detection: Distribution of catecholamines in the limbic system.

A method is described for the simultaneous quantification of dopamine, norepinephrine and epinephrine in microdissected rat brain nuclei using on-line trace enrichment high performance liquid chromatography (HPLC) and electrochemical detection (EC). The method is specific, sensitive and rapid with lower limits of detection comparable to those of radioenzymatic and mass fragmentographic techniques. The ideal application of the method to determinations in discrete microdissected brain nuclei is illustrated by mapping the topographic distribution of these three catecholamines in the limbic system of the rat. Dopamine is well represented and remarkably heterogeneously distributed in the amygdaloid nuclei. Norepinephrine concentrations are high in the interstitial (bed) nucleus of the stria terminalis and the paraventricular, periventricular, dorsomedial and medial preoptic hypothalamic nuclei, though generally more evenly distributed among the limbic nuclei than dopamine. Epinephrine was reliably quantified in a minority of the limbic nuclei examined with the highest concentration found in the paraventricular hypothalamic nuclei. The method described is ideally suited for examination of the pharmacological and functional heterogeneity of dopamine-, norepinephrine- and epinephrine-containing neuronal systems at the level of discrete brain nuclei.